Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

ColE1-type vectors with fully repressible replication.

D Gil1, J P Bouché

  • 1Centre de Recherche de Biochimie et de Génétique Cellulaires, CNRS, Toulouse, France.

Gene
|August 30, 1991
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Predicted no effect concentrations of antifungals for wastewater management and agricultural use.

Frontiers in toxicology·2026
Same author

Female Starlings With Experimentally Impaired Pre-Laying Condition Produce Smaller But Vitamin A Richer Eggs.

Journal of experimental zoology. Part A, Ecological and integrative physiology·2025
Same author

Time to match; when do homologous chromosomes become closer?

Chromosoma·2022
Same author

Chromosomal positioning in spermatogenic cells is influenced by chromosomal factors associated with gene activity, bouquet formation and meiotic sex chromosome inactivation.

Chromosoma·2021
Same author

Anti-inflammatory coating of hernia repair meshes: a 5-rabbit study.

Hernia : the journal of hernias and abdominal wall surgery·2020
Same author

Influence of fiber connectivity in simulations of cardiac biomechanics.

International journal of computer assisted radiology and surgery·2018
Same journal

TBX6 promotes proliferation, invasion, and migration in colorectal cancer: Integrated transcriptomic and protein interaction network analysis.

Gene·2026
Same journal

Face/off: phase-specific modeling of lineage plasticity using near-patient models in genitourinary cancers.

Gene·2026
Same journal

Hierarchical analysis of metabolic phenotype reveals distinct microbiota and circulatory transcriptome in metabolic dysfunction-associated steatotic liver disease.

Gene·2026
Same journal

Mutation T71R enhanced the structural stability and functional activity of wild type superoxide dismutase cloned from soil metagenome.

Gene·2026
Same journal

Reduced ATXN1 expression as an adverse prognostic indicator in Acute myeloid leukemia.

Gene·2026
Same journal

Constructing regulatory networks of Rubisco post-translational modifications: a novel avenue for engineering environment adaptive plants.

Gene·2026
See all related articles

New cloning vectors, pAM34 and pAM35, enable inducible plasmid replication. This allows for controlled maintenance, stable chromosomal integration, and recovery of adjacent DNA sequences, offering flexibility in genetic engineering applications.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Standard cloning vectors often lack precise control over replication.
  • The need for inducible replication systems is crucial for stable genetic manipulation and recovery of flanking sequences.

Purpose of the Study:

  • To construct novel cloning vectors (pAM34, pAM35) with inducible replication.
  • To enable controlled maintenance and chromosomal integration of plasmids.
  • To facilitate the recovery of nucleotide sequences adjacent to cloned fragments.

Main Methods:

  • Construction of pAM34 and pAM35 vectors derived from pBR322.
  • Incorporation of the lacZ promoter/operator for inducible transcription of replication primer RNA.
  • Inclusion of the lacIq gene in pAM34 for repressor-mediated induction control.

Related Experiment Videos

Main Results:

  • Replication of pAM34 and pAM35 is dependent on inducer addition when the lacIq repressor is present.
  • Vectors allow for temporary plasmid maintenance and stable chromosomal integration.
  • Successful recovery of nucleotide sequences adjacent to cloned fragments was demonstrated.

Conclusions:

  • The developed vectors offer precise control over plasmid replication through induction.
  • These tools are valuable for constructing strains with stable chromosomal vector integration.
  • The vectors facilitate efficient recovery of flanking DNA sequences, advancing genetic engineering strategies.