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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Overview Of Cell Separation And Isolation

Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.

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Related Experiment Video

Updated: Jun 24, 2026

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

On-chip microfluidic sorting with fluorescence spectrum detection and multiway separation.

Hirokazu Sugino1, Kazuto Ozaki, Yoshitaka Shirasaki

  • 1Laboratory of Bio-Analytical Chemistry, Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.

Lab on a Chip
|April 17, 2009
PubMed
Summary

This study introduces a microfluidic sorter for safe cell and material handling. The device achieves high accuracy and purity in separating diverse specimens using fluorescence spectrum detection.

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Last Updated: Jun 24, 2026

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Microfluidics

Background:

  • Microfluidic platforms are crucial for safe handling of precious cells and infectious materials in diagnostics and research.
  • Accurate and efficient separation of biological specimens is essential for various biomedical applications.

Purpose of the Study:

  • To develop an on-chip microfluidic sorter with integrated fluorescence spectrum detection.
  • To achieve multiway separation of different types of specimens based on their spectral characteristics.

Main Methods:

  • Development of a microfluidic chip with a 1x16 arrayed photomultiplier tube for rapid fluorescence spectrum acquisition (495-685 nm, 2 ms intervals).
  • Utilized a thermoreversible gelation polymer technique for specimen collection into designated channels.
  • Separation of four types of fluorescent microspheres and three types of fluorescent protein-expressing Escherichia coli cells.

Main Results:

  • Successful separation of diverse specimens, including fluorescent microspheres and E. coli cells, with accuracy and purity exceeding 90%.
  • Demonstrated a throughput of approximately one particle per second.
  • Validated the effectiveness of fluorescence spectrum detection for specimen identification and sorting.

Conclusions:

  • The developed on-chip microfluidic sorter offers a highly accurate and efficient method for specimen separation.
  • The integration of fluorescence spectrum detection with microfluidic sorting provides a powerful tool for biomedical studies and diagnostics.
  • The thermoreversible gelation polymer technique enables precise collection of sorted specimens.