Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Integrated circuit-microfluidic biosensors for blood-based disease diagnostics-A review.

Analytica chimica acta·2026
Same author

Biofunctional thermoresponsive gelatin-PNIPAm microcarriers with embedded zero-valent iron nanoparticles for enhanced human adipose-derived stem cell expansion and differentiation.

Biomaterials advances·2026
Same author

A modified digital workflow for fabricating tooth- and implant-supported conical crown-retained partial dentures using selective laser melting: A dental technique.

The Journal of prosthetic dentistry·2026
Same author

Running exercise mitigates amyloidosis in 5xFAD mice by improving the structure and function of the meningeal lymphatic system.

Acta neuropathologica communications·2026
Same author

Synergistic Sterilization via Dual-Wavelength LED: Reducing UV Energy and Enhancing Microbial Inactivation through Optimized Irradiation Sequencing.

IEEE transactions on bio-medical engineering·2026
Same author

Optimization of a microfluidic system for automated detection of Cholangiocarcinoma cells in bile.

Biosensors & bioelectronics·2025

Related Experiment Video

Updated: Jun 24, 2026

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture
09:51

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

Published on: June 16, 2016

A microfluidic cell culture platform for real-time cellular imaging.

Chia-Chun Hsieh1, Song-Bin Huang, Ping-Ching Wu

  • 1Department of Engineering Science, National Cheng Kung University, Tainan, Taiwan.

Biomedical Microdevices
|April 17, 2009
PubMed
Summary

Researchers developed a novel microfluidic cell culture platform for real-time cellular analysis. This automated system enables high-resolution imaging and drug screening, advancing in vitro cellular studies.

More Related Videos

Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation
12:04

Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation

Published on: December 6, 2013

Generation of Dynamical Environmental Conditions using a High-Throughput Microfluidic Device
14:48

Generation of Dynamical Environmental Conditions using a High-Throughput Microfluidic Device

Published on: April 17, 2021

Related Experiment Videos

Last Updated: Jun 24, 2026

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture
09:51

A Microfluidic Platform for High-throughput Single-cell Isolation and Culture

Published on: June 16, 2016

Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation
12:04

Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation

Published on: December 6, 2013

Generation of Dynamical Environmental Conditions using a High-Throughput Microfluidic Device
14:48

Generation of Dynamical Environmental Conditions using a High-Throughput Microfluidic Device

Published on: April 17, 2021

Area of Science:

  • Biotechnology
  • Microfluidics
  • Cell Biology

Background:

  • Traditional cell culture methods lack real-time monitoring capabilities.
  • In vitro studies require advanced platforms for precise cellular environment control.
  • Visualizing nanoparticle-cell interactions necessitates high-resolution imaging techniques.

Purpose of the Study:

  • To develop an automated microfluidic cell culture platform for real-time, in vitro cellular function evaluation.
  • To enable high-resolution imaging of cellular processes and nanoparticle-cell interactions.
  • To facilitate drug screening and investigate nano vector delivery in situ.

Main Methods:

  • Integration of microheaters, micro temperature sensor, and micropumps for a perfusion-based microenvironment.
  • Utilization of an ultra-thin culture chamber (180 µm depth) for enhanced imaging.
  • Combination of bright field and fluorescent microscopy for detailed visualization.
  • Automated processes for cell plating, culturing, harvesting, and replenishing.

Main Results:

  • Successful real-time monitoring of cellular functions and mitotic activity.
  • Demonstrated visualization of nanoparticle-cell/organelle interactions.
  • Enabled in situ investigation of nano vector delivery and cellular uptake.
  • Validated drug screening capabilities using conjugated quantum dots-epirubicin.

Conclusions:

  • The developed microfluidic platform offers a self-contained, automated solution for advanced cell culture.
  • It provides high-resolution imaging for detailed analysis of cellular dynamics and drug delivery.
  • The system holds significant potential for high-throughput cell-based assays and diagnostic cellular imaging.