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Related Concept Videos

Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
The...
Two-Dimensional Microscopy in Microbiology01:29

Two-Dimensional Microscopy in Microbiology

Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...
FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Photoluminescence: Applications01:14

Photoluminescence: Applications

Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...

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Related Experiment Video

Updated: Jun 23, 2026

Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging
10:55

Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging

Published on: January 5, 2015

Fluorescence as a tool for advanced diagnostics.

M Hazell1

  • 1Cambridge Consultants Ltd, Cambridge, UK. info@cambridgeconsultants.com

Medical Device Technology
|April 18, 2009
PubMed
Summary
This summary is machine-generated.

Fluorescence is a versatile technique used in medical diagnostics, from simple over-the-counter tests to advanced clinical analyzers. Future applications promise further innovation in this powerful analytical method.

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Last Updated: Jun 23, 2026

Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging
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Published on: January 5, 2015

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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Medical Diagnostics

Background:

  • Fluorescence is a widely applicable analytical technique.
  • It is utilized in various medical settings, from point-of-care devices to sophisticated clinical analyzers.

Purpose of the Study:

  • To describe the technique of fluorescence.
  • To illustrate the versatility of fluorescence through diverse examples.
  • To explore future prospects of fluorescence technology.

Main Methods:

  • Literature review and synthesis of existing knowledge on fluorescence.
  • Case studies and examples showcasing fluorescence applications.
  • Trend analysis and future outlook prediction for fluorescence techniques.

Main Results:

  • Fluorescence finds application in a broad spectrum of diagnostic tools.
  • Examples demonstrate its utility in both low-cost and high-complexity analytical systems.
  • The technique's adaptability supports its widespread use in clinical settings.

Conclusions:

  • Fluorescence is a powerful and versatile technique with a significant impact on medical diagnostics.
  • Its adaptability ensures continued relevance and development in various healthcare applications.
  • The future of fluorescence in analytical and clinical settings appears promising for further advancements.