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08:01
Tuning a Parallel Segmented Flow Column and Enabling Multiplexed Detection
Published on: December 15, 2015
Examination of the temporal effect in a flow injection analysis system using multi-channel absorbance detection.
Shu-Hui Lin1, Kai-Chieh Chang, Yu-Chun Chen
1Department of Applied Chemistry, National Chiao Tung University, Hsinchu, Taiwan.
Journal of Chromatography. A
|April 18, 2009
Summary
A new temporal effect explains tailing peaks in flow injection analysis (FIA). This effect, more significant than the Poiseuille effect at higher flow rates, intensifies with increased flow.
Area of Science:
- Analytical Chemistry
- Chromatography and Separation Science
Background:
- Flow Injection Analysis (FIA) commonly exhibits peak tailing.
- The Poiseuille effect is a known contributor to peak shape.
- A recent 'temporal effect' has been proposed to explain residual peak tailing.
Purpose of the Study:
- To investigate the proposed temporal effect in FIA.
- To determine the contribution of the temporal effect to peak tailing.
- To analyze the influence of flow rate on the temporal effect.
Main Methods:
- Utilized a multi-channel absorbance detector to capture FIA peaks in spatial and temporal domains.
- Compared experimental peak profiles with Gaussian peaks.
- Assessed asymmetry factors between spatial and temporal peak data.
Main Results:
- The temporal effect was identified as the primary cause of peak tailing across flow rates of 0.5–8 mL/min.
- A spatially advanced peak in FIA tubing correlated with temporal peak tailing.
- The magnitude of the temporal effect increased with higher flow rates.
Conclusions:
- The temporal effect is a significant factor in FIA peak tailing, particularly at elevated flow rates.
- Understanding the temporal effect is crucial for accurate FIA signal interpretation.
- Flow rate optimization is necessary to mitigate temporal effect-induced peak distortion.
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