Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Staphylococcal Skin Infections01:29

Staphylococcal Skin Infections

Staphylococcus aureus is a Gram-positive coccus that resides harmlessly on the skin and mucous membranes of healthy individuals. When the skin barrier is breached, it can shift from a commensal to an opportunistic pathogen. This transition is facilitated by surface adhesins, such as clumping factor B and S. aureus surface protein G (SasG), which bind to structural proteins, including loricrin and cytokeratin, in the damaged epidermis. Protein A, another key factor, binds the Fc region of...
Mechanism of Antibiotic Resistance in MRSA01:25

Mechanism of Antibiotic Resistance in MRSA

Antibiotic resistance in bacteria arises when microorganisms evolve the ability to withstand drugs designed to kill them or inhibit their growth, rendering once-effective treatments useless. This phenomenon, driven by genetic change and selection under antibiotic exposure, poses a profound threat to modern medicine. Mechanisms include drug-inactivating enzymes (e.g., β-lactamases), efflux pumps that eject antibiotics, mutations altering antibiotic targets, decreased drug uptake, and acquisition...
Clinical Significance of Antibiotic Resistance01:25

Clinical Significance of Antibiotic Resistance

Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within the One...
Determinants of Bacterial Pathogenicity and Virulence01:20

Determinants of Bacterial Pathogenicity and Virulence

Pathogenic bacteria employ a variety of strategies to establish infections, including the secretion of extracellular enzymes that act as potent virulence factors. These enzymes facilitate bacterial colonization of host tissues and help evade immune surveillance. By targeting structural components of host tissues and interfering with immune mechanisms, these enzymes play a pivotal role in disease progression.Extracellular Enzymes Facilitating Tissue Invasion: Several bacterial pathogens secrete...
Surface Appendages of Archaea01:23

Surface Appendages of Archaea

Archaeal surface appendages are highly specialized structures essential for environmental adaptation, encompassing roles in adhesion, biofilm formation, and motility. Among these appendages, pili and archaella stand out for their distinct morphologies and functionalities, enabling archaea to thrive in diverse and often extreme environments.Pili: Adhesion and Biofilm FormationPili are filamentous structures assembled from pilin protein subunits, primarily contributing to adhesion and biofilm...
Streptococcal Pharyngitis01:27

Streptococcal Pharyngitis

Streptococcal pharyngitis, commonly known as “strep throat,” is an acute infection of the oropharyngeal tissues caused by the Gram‑positive Group A Streptococcus (Streptococcus pyogenes). Transmission occurs primarily through respiratory droplets expelled during coughing, sneezing, or talking.Mechanisms of Host Entry and Immune EvasionUpon entering the host, S. pyogenes adheres to the mucosal epithelial cells of the pharynx via surface proteins, notably lipoteichoic acid and the antiphagocytic...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

European Association of Urology Guidelines on Urological Infections: Summary of the 2026 Guidelines.

European urology·2026
Same author

EAU-EANM-ESTRO-ESUR-ISUP-SIOG Guidelines on Prostate Cancer-2026 Update. Part I: Screening, Diagnosis, and Local Treatment with Curative Intent.

European urology·2026
Same author

EAU-EANM-ESTRO-ESUR-ISUP-SIOG Guidelines on Prostate Cancer. Part II-2026 Update: Treatment of Relapsing and Metastatic Prostate Cancer.

European urology·2026
Same author

EAU Guidelines on Nonmuscle-invasive Bladder Cancer (TaT1 and CIS) - A Summary of the 2026 Guidelines Update.

European urology·2026
Same author

Plasminogen Recruitment by <i>Staphylococcus aureus</i> SdrC Reveals a Tractable Antivirulence Target.

ACS infectious diseases·2026
Same author

Simulated wildfire smoke particulate matter elicits STING-dependent astrocyte-mediated inflammatory signaling and neurotoxicity.

Neurotoxicology·2026

Related Experiment Video

Updated: Jun 23, 2026

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues
07:10

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues

Published on: February 19, 2019

Staphylococcus pseudintermedius expresses surface proteins that closely resemble those from Staphylococcus aureus.

Joan A Geoghegan1, Emma J Smith, Pietro Speziale

  • 1Microbiology Department, Moyne Institute of Preventive Medicine, Trinity College, Dublin, Ireland.

Veterinary Microbiology
|April 18, 2009
PubMed
Summary

Staphylococcus pseudintermedius expresses surface proteins similar to Staphylococcus aureus, including those that bind fibrinogen and fibronectin. These findings suggest potential roles in canine pyoderma pathogenesis and host colonization.

More Related Videos

Development and Assessment of Intracellular Infection Models for Staphylococcus aureus
08:32

Development and Assessment of Intracellular Infection Models for Staphylococcus aureus

Published on: January 17, 2025

Related Experiment Videos

Last Updated: Jun 23, 2026

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues
07:10

A Fluorescence-based Method to Study Bacterial Gene Regulation in Infected Tissues

Published on: February 19, 2019

Development and Assessment of Intracellular Infection Models for Staphylococcus aureus
08:32

Development and Assessment of Intracellular Infection Models for Staphylococcus aureus

Published on: January 17, 2025

Area of Science:

  • Microbiology
  • Veterinary Dermatology
  • Molecular Biology

Background:

  • Staphylococcus pseudintermedius is a common dog commensal bacteria.
  • It is frequently involved in the development of canine pyoderma.
  • Surface protein expression in S. pseudintermedius is not well understood.

Purpose of the Study:

  • To investigate if S. pseudintermedius expresses surface proteins analogous to those found in Staphylococcus aureus.
  • To characterize these potential surface proteins and their binding capabilities.

Main Methods:

  • Adhesion assays using purified fibrinogen, fibronectin, and cytokeratin 10.
  • Ligand-affinity blotting and Western blotting of cell-wall extracts.
  • Genomic DNA probing using S. aureus clfA gene sequences.

Main Results:

  • S. pseudintermedius strain 326 adhered strongly to fibrinogen, fibronectin, and cytokeratin 10.
  • A cell-wall anchored fibronectin-binding protein was identified.
  • A 180kDa protein binding fibrinogen and cross-reacting with antibodies against S. aureus ClfA and SdrD was detected.

Conclusions:

  • S. pseudintermedius possesses surface proteins with functional similarities to S. aureus virulence factors.
  • These proteins, particularly those binding fibrinogen and fibronectin, may contribute to S. pseudintermedius pathogenesis in dogs.
  • Further research is needed to confirm the identity and function of the cytokeratin-binding protein.