Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Multi-species Conserved Sequences02:51

Multi-species Conserved Sequences

Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale  studies have provided new insights into the evolutionary relationship between organisms.
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved DNA...
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
Histone Variants at the Centromere02:30

Histone Variants at the Centromere

Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3 variants are also...
Exon Recombination02:32

Exon Recombination

The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Non-LTR Retrotransposons03:18

Non-LTR Retrotransposons

As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
LTR Retrotransposons03:08

LTR Retrotransposons

LTR retrotransposons are class I transposable elements with long terminal repeats flanking an internal coding region. These elements are less abundant in mammals compared to other class I transposable elements. About 8 percent of human genomic DNA comprises LTR retrotransposons. Some of the common examples of LTR retrotransposons are Ty elements in yeast and Copia elements in Drosophila.
The internal coding region of LTR retrotransposons and their mechanism of transposition closely resembles a...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mutational analysis of the putative fusion domain of Ebola virus glycoprotein.

Journal of virology·1999
Same author

Activities of new antimicrobial agents (trovafloxacin, moxifloxacin, sanfetrinem, and quinupristin-dalfopristin) against Bacteroides fragilis group: comparison with the activities of 14 other agents.

Antimicrobial agents and chemotherapy·1999
Same author

Hyperenhancing focal liver lesions: differential diagnosis with helical CT.

AJR. American journal of roentgenology·1999
Same author

Formulation strategies for the stabilization of tetanus toxoid in poly(lactide-co-glycolide) microspheres.

International journal of pharmaceutics·1999
Same author

Post-weaning multisystemic wasting syndrome in Spain.

The Veterinary record·1999
Same author

[Congenital defects of antithrombin III and proteins C and S during pregnancy].

Revista medica de Chile·1999

Related Experiment Video

Updated: Jun 23, 2026

An Integrated Approach for Microprotein Identification and Sequence Analysis
09:37

An Integrated Approach for Microprotein Identification and Sequence Analysis

Published on: July 12, 2022

A new pericentromeric repeated DNA sequence in Microtus thomasi.

M J Acosta1, J A Marchal, G P Mitsainas

  • 1Departamento de Biología Experimental, Facultad de Ciencias Experimentales, Universidad de Jaén, Jaén, Spain.

Cytogenetic and Genome Research
|April 18, 2009
PubMed
Summary

Researchers identified a novel repeated DNA sequence, Mth-Alu900, in the Greek vole Microtus thomasi. This AT-rich sequence is specific to M. thomasi and located in heterochromatin, offering insights into vole genome evolution.

More Related Videos

Dissection of Hippocampal Dentate Gyrus from Adult Mouse
07:42

Dissection of Hippocampal Dentate Gyrus from Adult Mouse

Published on: November 17, 2009

Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark
10:09

Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark

Published on: January 26, 2018

Related Experiment Videos

Last Updated: Jun 23, 2026

An Integrated Approach for Microprotein Identification and Sequence Analysis
09:37

An Integrated Approach for Microprotein Identification and Sequence Analysis

Published on: July 12, 2022

Dissection of Hippocampal Dentate Gyrus from Adult Mouse
07:42

Dissection of Hippocampal Dentate Gyrus from Adult Mouse

Published on: November 17, 2009

Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark
10:09

Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark

Published on: January 26, 2018

Area of Science:

  • Genetics
  • Molecular Biology
  • Evolutionary Biology

Background:

  • Populations of the vole species Microtus thomasi in Greece exhibit diverse karyotypic forms.
  • Distinct karyomorphs, M. thomasi 'thomasi' and M. thomasi 'atticus', differ in X chromosome morphology (acrocentric vs. subtelocentric).
  • Significant variability in heterochromatin content of sex chromosomes is noted in both karyomorphs.

Purpose of the Study:

  • To clone and characterize a repeated DNA sequence from M. thomasi 'atticus'.
  • To investigate the chromosomal location and distribution of this novel sequence in different M. thomasi karyomorphs.
  • To understand the role of this sequence in the context of karyotypic and sex chromosome variations within M. thomasi.

Main Methods:

  • Genomic DNA digestion using AluI enzyme.
  • Cloning and sequencing of an 884 bp repeated DNA sequence (Mth-Alu900).
  • Analysis of chromosomal location using techniques on M. thomasi 'thomasi' and M. thomasi 'atticus' karyomorphs.

Main Results:

  • An AT-rich, 884 bp repeated DNA sequence, Mth-Alu900, was successfully cloned from M. thomasi 'atticus'.
  • Mth-Alu900 is composed primarily of three simple repeats (CAAAT, CAGAT, CAGAC) and appears organized as a dimer.
  • This sequence is exclusive to M. thomasi and predominantly located in the pericentromeric heterochromatin of autosomes and X chromosomes in both studied karyomorphs.
  • Mth-Alu900 represents a new pericentromeric repeated DNA sequence discovered in Microtus species.

Conclusions:

  • The Mth-Alu900 sequence is a novel, M. thomasi-specific repeated DNA element.
  • Its pericentromeric localization suggests a role in chromosome structure and potentially in the karyotypic variations observed in M. thomasi.
  • This finding contributes to the understanding of genome organization and evolution in the Microtus genus.