Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Serum Biomarkers of Macrophage Activation and Incident CKD in People With and Without HIV: Findings From the MACS-WIHS Combined Cohort Study.

Open forum infectious diseases·2026
Same author

Role of biomarkers in predicting disease severity in acute dengue and SARs-CoV-2-Infected patients.

BMC infectious diseases·2025
Same author

Effect of Pitavastatin on Epigenetic Aging Biomarkers in People With HIV: Pilot Substudy of the REPRIEVE Trial.

Clinical infectious diseases : an official publication of the Infectious Diseases Society of America·2025
Same author

Glycation metabolites predict incident age-related comorbidities and mortality in older people with HIV.

GeroScience·2025
Same author

Opposite Roles of IL-32α Versus IL-32β/γ Isoforms in Promoting Monocyte-Derived Osteoblast/Osteoclast Differentiation and Vascular Calcification in People with HIV.

Cells·2025
Same author

Piloting an event-based surveillance model in private hospitals for early detection of disease clusters, Kerala, India.

The Indian journal of medical research·2025

Related Experiment Video

Updated: Jun 23, 2026

Comprehensive & Cost Effective Laboratory Monitoring of HIV/AIDS: an African Role Model
23:56

Comprehensive & Cost Effective Laboratory Monitoring of HIV/AIDS: an African Role Model

Published on: October 31, 2010

Advances in CD4 cell enumeration in resource-poor countries.

Linda L Baum1, Suzanne Crowe, Alan L Landay

  • 1aRush University Medical Center, Chicago, Illinois, USA bMacfarlane Burnet Institute for Medical Research and Public Health, Melbourne, Victoria, Australia.

Current Opinion in HIV and AIDS
|April 18, 2009
PubMed
Summary

Rapid CD4 cell assays are crucial for HIV treatment in resource-poor settings. New, cost-effective, and portable methods are emerging to improve monitoring and therapy efficacy.

More Related Videos

The α-test: Rapid Cell-free CD4 Enumeration Using Whole Saliva
11:22

The α-test: Rapid Cell-free CD4 Enumeration Using Whole Saliva

Published on: May 16, 2012

Quantification of Proliferating Human Antigen-specific CD4+ T Cells using Carboxyfluorescein Succinimidyl Ester
07:00

Quantification of Proliferating Human Antigen-specific CD4+ T Cells using Carboxyfluorescein Succinimidyl Ester

Published on: June 4, 2019

Related Experiment Videos

Last Updated: Jun 23, 2026

Comprehensive & Cost Effective Laboratory Monitoring of HIV/AIDS: an African Role Model
23:56

Comprehensive & Cost Effective Laboratory Monitoring of HIV/AIDS: an African Role Model

Published on: October 31, 2010

The α-test: Rapid Cell-free CD4 Enumeration Using Whole Saliva
11:22

The α-test: Rapid Cell-free CD4 Enumeration Using Whole Saliva

Published on: May 16, 2012

Quantification of Proliferating Human Antigen-specific CD4+ T Cells using Carboxyfluorescein Succinimidyl Ester
07:00

Quantification of Proliferating Human Antigen-specific CD4+ T Cells using Carboxyfluorescein Succinimidyl Ester

Published on: June 4, 2019

Area of Science:

  • Clinical diagnostics
  • Immunology
  • Public health

Background:

  • Antiretroviral therapy for HIV requires CD4 cell count monitoring.
  • Resource-poor countries face challenges in accessing reliable CD4 testing.

Purpose of the Study:

  • To review advancements in rapid CD4 cell assay development.
  • To evaluate assays for HIV treatment in resource-limited settings.

Main Methods:

  • Review of recent progress in rapid CD4 cell assay development and evaluation.
  • Analysis of field test data for simplified flow-based and manual assays.
  • Assessment of emerging technologies like microchip digital imaging and immunochromatography.

Main Results:

  • Simplified flow-based assays show comparable results to standard methods at lower costs.
  • Portable and manual assay options are available for different testing volumes.
  • New technologies aim for reduced cost, enhanced portability, and ease of use.
  • Reagents for improved sample and reagent stability in tropical climates are under evaluation.

Conclusions:

  • The need for CD4 cell assays is increasing with wider access to antiretroviral medications.
  • Simplified flow cytometry is suitable for centralized clinics.
  • A critical need exists for more affordable and simpler methods for rural and small laboratories.