Related Experiment Video
Updated: Jun 23, 2026
Biobanking of Human Aqueous and Vitreous Liquid Biopsies for Molecular Analyses
Published on: September 11, 2023
A prospective study of in-office diagnostic vitreous sampling in patients with vitreoretinal pathology
Scott M Pfahler1, Angela N Brandford, Bert M Glaser
1National Retina Institute, Towson, Maryland 21204, USA.
Purpose:
To evaluate the safety and efficacy of in-office vitreous sampling (50-100 microL).
Methods:
A prospective, consecutive case series of 578 diagnostic vitreous sample attempts performed between February 23, 2007 and September 31, 2008. A standardized technique of vitreous sampling followed by intravitreal injection of therapeutic medication was performed. All subjects were followed for adverse events after sampling/injection.
Results:
Out of a total of 578 vitreous sampling attempts, a total of 550 positive diagnostic vitreous samples were collected from 157 eyes of 144 patients. There were a total of 28 "dry" samples in 19 subjects (13 females and 6 males). The rate of dry sample was 4.8% (28 of 578). Complications included transient vitreous hemorrhage (n = 3), transient intraocular inflammation (n = 3), localized, chronic retinal detachment (n = 1). None of the complications resulted in permanent visual sequelae. These complications were no different than those expected from intravitreal injection alone.
Conclusion:
This prospective study has shown that in-office vitreous sampling of 50 microL to 100 microL is a safe and reproducible procedure. Larger randomized studies will be helpful to further assess the safety and long-term effects of in-office diagnostic vitreous sampling.
