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Related Experiment Video

Updated: Jun 23, 2026

A Generalized Method for Determining Free Soluble Phenolic Acid Composition and Antioxidant Capacity of Cereals and Legumes
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Improved method of total antioxidant assay.

Ruby Gupta1, Mukta Sharma, Ramakrishnan Lakshmy

  • 1Department of Cardiac Biochemistry, All India Institute of Medical Sciences, Ansari Nagar, New Delhi 110029.

Indian Journal of Biochemistry & Biophysics
|April 21, 2009
PubMed
Summary

This study presents a cost-effective microplate assay for measuring total antioxidant status. The adapted method allows for higher sample throughput and reduced reagent use compared to traditional spectrophotometric methods.

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Biomedical Science

Background:

  • Commercially available assays for total antioxidant status are often expensive and labor-intensive.
  • Current methods require strict control of incubation time and temperature (37°C).
  • Traditional assays limit sample analysis to approximately 40 samples per day.

Purpose of the Study:

  • To adapt an existing total antioxidant status assay to a microplate format.
  • To reduce assay costs and increase the number of samples that can be analyzed daily.
  • To validate the reliability of the adapted microplate assay.

Main Methods:

  • Adapted a standard spectrophotometric assay for total antioxidant status to a microplate reader.
  • Reduced reagent volume to one-fourth of the original procedure.
  • Analyzed 30 serum samples using both the original spectrophotometric method and the adapted microplate assay.

Main Results:

  • The microplate assay allows for a higher number of samples to be analyzed per day.
  • Reagent consumption was reduced by 75% compared to the original method.
  • Total antioxidant values obtained from the microplate assay showed good correlation with the original spectrophotometric method.

Conclusions:

  • The adapted microplate assay provides a reliable and cost-effective method for estimating total antioxidant status.
  • This microplate adaptation significantly increases daily sample throughput.
  • The method offers a practical alternative for routine laboratory analysis of antioxidant capacity.