Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Cellulose and Pectic Polysaccharides

Cellulose and Pectic Polysaccharides

Every plant cell has a cell wall that protects the cell, provides structural support, and gives the cell shape. Cellulose, the main structural component of the plant cell wall, makes up over 30% of plant matter. It is the most abundant organic compound on earth. Cellulose is an unbranched polysaccharide composed of linear chains of glucose molecules linked by β (1→4) glycosidic bonds.
As a cell matures, its cell wall specializes according to its type. For example, the parenchyma cells of...

Atomic Force Microscopy

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

[Operation preparation in trauma surgery].

Der Unfallchirurg·2021

Same author

Horizontal drying fronts in films of colloidal dispersions: influence of hydrostatic pressure and collective diffusion.

Soft matter·2017

Same author

Velocity of lateral drying fronts in film formation by drying of colloidal dispersions. A 2D simulation.

Journal of colloid and interface science·2017

Same author

Simulation of Vertical Surfactant Distributions in Drying Latex Films.

Langmuir : the ACS journal of surfaces and colloids·2016

Same author

Drying Mechanisms in Plasticized Latex Films: Role of Horizontal Drying Fronts.

The journal of physical chemistry. B·2016

Same author

Simulation of Latex Film Formation Using a Cell Model in Real Space: Vertical Drying.

Langmuir : the ACS journal of surfaces and colloids·2015

Search research articles

Related Experiment Video

Updated: Jun 23, 2026

Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy

Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy

Published on: July 10, 2019

Interfacial structure of sugar beet pectin studied by atomic force microscopy.

A Gromer¹, A R Kirby, A P Gunning

¹Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, UK. axel.gromer@bbrsc.ac.uk

Langmuir : the ACS Journal of Surfaces and Colloids

|April 21, 2009

Summary

Sugar beet pectin (SBP) forms unique interfacial structures due to its protein content, showing enhanced stability against surfactants. Understanding this nanoscale organization can improve SBP

More Related Videos

Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy

Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy

Published on: August 20, 2018

Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping

Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping

Published on: October 24, 2014

Related Experiment Videos

Last Updated: Jun 23, 2026

Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy

Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy

Published on: July 10, 2019

Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy

Covalent Immobilization of Proteins for the Single Molecule Force Spectroscopy

Published on: August 20, 2018

Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping

Atomic Force Microscopy of Red-Light Photoreceptors Using PeakForce Quantitative Nanomechanical Property Mapping

Published on: October 24, 2014

Area of Science:

Food science and technology
Materials science
Biochemistry

Background:

Sugar beet pectin (SBP) exhibits unique emulsifying properties attributed to its hydrophobicity and high protein content.
Investigating SBP's interfacial structure is crucial for understanding its functionality in emulsions.

Purpose of the Study:

To elucidate the nanoscale structure of sugar beet pectin (SBP) at various interfaces.
To understand the role of proteins and pectin chains in SBP's interfacial behavior.
To correlate interfacial structure with SBP's emulsifying properties.

Main Methods:

Atomic force microscopy (AFM) to visualize SBP structure at mica/water, graphite/water, and air/water interfaces.
Langmuir-Blodgett method for transferring interfacial films onto mica.
Pendant drop and surface shear rheology to assess interfacial film elasticity.
Comparison with alkali-treated SBP and pure protein films.

Main Results:

SBP forms distinct layers on graphite, potentially via CH-pi interactions, and elastic films at the air/water interface.
AFM revealed holes and rod-like chains in the SBP interfacial film, indicating pectin chains hinder dense protein packing.
SBP interfacial films demonstrated superior resistance to surfactant displacement compared to pure protein films.
Alkali treatment removed pectin chains, resulting in a protein-like interfacial film.

Conclusions:

SBP's nanoscale interfacial organization, influenced by protein-pectin interactions, dictates its functional properties.
The unique structure of SBP at interfaces contributes to its enhanced stability and emulsifying capabilities.
This research provides insights for rationally modifying SBP to optimize its performance in commercial applications.