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Updated: Jun 23, 2026

Mass Spectrometry-Guided Genome Mining as a Tool to Uncover Novel Natural Products
Published on: March 12, 2020
Chapter 23. Cyanobactin ribosomally synthesized peptides--a case of deep metagenome mining
Eric W Schmidt1, Mohamed S Donia
1Department of Medicinal Chemistry, University of Utah, Salt Lake City, Utah, USA.
Abstract:
Deep metagenome mining is a new method for engineering natural product pathways, focusing on examining symbiotic organisms. The method has been applied to a family of compounds known as cyanobactins, which are ribosomally synthesized peptides produced by cyanobacteria. Often, these cyanobacteria live symbiotically with marine animals, leading to production of natural products in whole animal samples. Here, we focus on methods to identify, clone, and study cyanobactin natural product genes from axenic organisms and metagenomic environments. The application to deep metagenome mining is described, along with other potential targets of this methodology.
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