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Rolling-circle amplification of viral DNA genomes using phi29 polymerase

Reimar Johne1, Hermann Müller, Annabel Rector

  • 1Federal Institute for Risk Assessment, Diedersdorfer Weg 1, 12277 Berlin, Germany. Reimar.Johne@bfr.bund.de

Trends in Microbiology
|April 21, 2009
PubMed
Summary

Rolling-circle amplification (RCA) using bacteriophage phi29 DNA polymerase enables efficient whole-genome amplification. This technique is valuable for studying viral genomes in diverse applications, including diagnostics and infectious clone generation.

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