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Related Experiment Video

Updated: Jun 23, 2026

A Phenotyping Regimen for Genetically Modified Mice Used to Study Genes Implicated in Human Diseases of Aging
09:37

A Phenotyping Regimen for Genetically Modified Mice Used to Study Genes Implicated in Human Diseases of Aging

Published on: July 14, 2016

Smad2 isoforms are differentially expressed during mouse brain development and aging.

Uwe Ueberham1, Peggy Lange, Elke Ueberham

  • 1Department for Molecular and Cellular Mechanisms of Neurodegeneration, University of Leipzig, Paul Flechsig Institute of Brain Research, Leipzig 04109, Germany. Uwe.Ueberham@medizin.uni-leipzig.de

International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience
|April 21, 2009
PubMed
Summary
This summary is machine-generated.

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Smad2(Deltaexon3), a Smad2 isoform, is abundant in the mouse brain and its expression changes with development and aging. This isoform is primarily found in neuronal nuclei, suggesting a key role in brain development and function.

Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genetics

Background:

  • Smad2 and Smad3 are key transcription factors in TGF-beta signaling, crucial for brain development.
  • These molecules are implicated in neurological disorders like Alzheimer's disease.
  • Smad2(Deltaexon3) is a splice variant with DNA-binding properties similar to Smad3.

Purpose of the Study:

  • To investigate the expression patterns of Smad2 isoforms in the mouse brain.
  • To determine the role of Smad2(Deltaexon3) in neuronal function and brain development.

Main Methods:

  • Quantitative analysis of Smad2 isoform expression in mouse brain.
  • Single-cell PCR to confirm neuronal expression of Smad2(Deltaexon3).
  • Subcellular fractionation to determine the localization of Smad2(Deltaexon3) in neurons.

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Dissection of Hippocampal Dentate Gyrus from Adult Mouse
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Last Updated: Jun 23, 2026

A Phenotyping Regimen for Genetically Modified Mice Used to Study Genes Implicated in Human Diseases of Aging
09:37

A Phenotyping Regimen for Genetically Modified Mice Used to Study Genes Implicated in Human Diseases of Aging

Published on: July 14, 2016

Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications
09:29

Isolation of Intermediate Filament Proteins from Multiple Mouse Tissues to Study Aging-associated Post-translational Modifications

Published on: May 18, 2017

Dissection of Hippocampal Dentate Gyrus from Adult Mouse
07:42

Dissection of Hippocampal Dentate Gyrus from Adult Mouse

Published on: November 17, 2009

Main Results:

  • Smad2(Deltaexon3) is the predominant Smad2 isoform in the adult mouse brain.
  • Smad expression patterns change during brain development and aging.
  • Smad2(Deltaexon3) is localized to the nuclear fraction of neurons, indicating a role in transcriptional regulation.

Conclusions:

  • Smad2(Deltaexon3) plays a significant role in neuronal function and brain development.
  • The distinct expression and localization of Smad2(Deltaexon3) suggest a specialized function in neurons.
  • Further research into Smad2(Deltaexon3) may reveal new therapeutic targets for neurological disorders.