Related Experiment Video
Updated: Aug 8, 2026
12:24
Murine Model of CD40-activation of B cells
Published on: March 6, 2010
Characterization of CD44 antigen during lymphoid ontogeny
A Collado1, A de Andres, E Cañadas
1Servicio de Análisis Clínicos e Inmunología, Hospital Virgen de las Nieves, Universidad de Granada, Spain.
Immunobiology
|September 1, 1991
Summary
The CD44 molecule is expressed on mature B cells but not on proliferating or pre-B cells. Protein kinase C activation drives B cell differentiation without affecting CD44 antigen expression.
Area of Science:
- Immunology
- Molecular Biology
- Cell Biology
Background:
- The CD44 molecule is a cell surface glycoprotein involved in cell adhesion and signaling.
- Its expression patterns are crucial for understanding lymphocyte development and function.
Purpose of the Study:
- To characterize the CD44 molecule using a newly produced monoclonal antibody (mAb), GRHL-1.
- To analyze the expression of CD44 in B lineage cells and its regulation during differentiation.
Main Methods:
- Immunohistological analysis of human tissues using GRHL-1 mAb.
- Biochemical analysis of 125I-labeled lymphocyte lysates via SDS-PAGE.
- Investigating the role of Protein Kinase C (PKC) in CD44 expression during B cell differentiation.
Main Results:
- GRHL-1 mAb demonstrated comparable reactivity to other CD44 cluster antibodies across various human tissues.
- CD44 expression was observed in mature B cells but absent in pre-B and Burkitt cell lines.
- CD44 was downregulated in proliferating B cells within germinal centers.
- PKC activation induced in vitro differentiation of pre-B cells but did not upregulate CD44 expression.
Conclusions:
- The GRHL-1 mAb is a reliable tool for studying CD44 expression.
- CD44 expression is a marker of mature B cell phenotype and is regulated during B cell proliferation and differentiation.
- PKC-mediated differentiation of B cells occurs independently of CD44 upregulation.
Related Concept Videos
01:18Lymphoid Cells and Tissues
Lymphoid cells and tissues are integral to the immune system, which is crucial in maintaining our body's defense against harmful pathogens. They form the building blocks of lymphoid organs, which include the spleen, thymus, and lymph nodes.
Lymphoid cells consist of various types of immune system cells. These include B and T lymphocytes, which are responsible for producing antibodies and killing infected cells, respectively. Dendritic cells act as messengers between the innate and adaptive...
Lymphoid cells consist of various types of immune system cells. These include B and T lymphocytes, which are responsible for producing antibodies and killing infected cells, respectively. Dendritic cells act as messengers between the innate and adaptive...
01:20Special Features of Adaptive Immunity
The adaptive immune system, a crucial component of the overall immune response, offers a highly specialized defense against pathogens. It involves specific cell types and features, enabling it to combat infections effectively and efficiently.
The primary cell types involved in adaptive immunity are T cells and B cells. Each type has a unique role in defending the body against pathogens. T cells are responsible for cell-mediated immunity. They identify and eliminate infected cells directly,...
The primary cell types involved in adaptive immunity are T cells and B cells. Each type has a unique role in defending the body against pathogens. T cells are responsible for cell-mediated immunity. They identify and eliminate infected cells directly,...
01:23Cells of the Adaptive Immune Response
The T and B lymphocytes of the adaptive immune system develop from common lymphoid progenitor cells in the bone marrow. These progenitors give rise to precursors that eventually develop into both T and B lymphocytes. As these precursors mature, they gain the ability to detect and respond to foreign antigens in the body, a process known as immunocompetence. Additionally, these precursors acquire self-tolerance, a process that ensures they do not react to self-antigens. This intricate system...
01:28Diversity of Antigen Receptors
Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
01:22T Cell Activation and Clonal Selection
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
Naive T cells that have not yet encountered an antigen express two primary CD...
01:24B Cell Activation and Differentiation
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...