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Updated: Aug 9, 2026
08:14
Isolation and Flow Cytometric Characterization of Murine Small Intestinal Lymphocytes
Published on: May 8, 2016
A role for intestinal T lymphocytes in bronchus mucosal immunity
F J Wallace1, A W Cripps, R L Clancy
1Auspharm Institute of Mucosal Immunology, Jesmond, Australia.
Immunology
|September 1, 1991
Summary
Intra-Peyer
Area of Science:
- Immunology
- Microbiology
- Respiratory Medicine
Background:
- Non-typable Haemophilus influenzae (NTHI) infections pose a significant respiratory health challenge.
- Intra-Peyer's patch (IPP) immunization with NTHI enhances bacterial clearance from the respiratory tract in rats.
- The precise mechanism behind this enhanced pulmonary clearance following mucosal immunization requires elucidation.
Purpose of the Study:
- To investigate the immunological mechanisms responsible for accelerated NTHI clearance from the respiratory tract after IPP immunization.
- To determine the relative contributions of intestinal T cells and specific antibodies to protective immunity against NTHI in the lungs.
Main Methods:
- Rats were immunized via IPP with NTHI.
- Mucosally derived lymphocytes (thoracic duct lymphocytes, TDL) and splenic lymphocytes from immunized rats were adoptively transferred to recipient rats.
- Bacterial clearance rates in the respiratory tract were measured in recipient rats after challenge with NTHI.
- The effect of antibody opsonization on NTHI clearance was assessed.
Main Results:
- Adoptive transfer of TDL or splenic lymphocytes from NTHI-immunized rats significantly accelerated bacterial clearance in recipient rats compared to controls.
- Transfer of TDL enriched for T lymphocytes also enhanced NTHI clearance.
- NTHI opsonized with specific antibodies did not show enhanced clearance in naive rats, indicating a limited role for antibody-dependent phagocytosis.
- These findings highlight the critical role of T cell-mediated immunity.
Conclusions:
- Immune clearance of NTHI from the respiratory tract following gut immunization is primarily mediated by antigen-primed lymphocytes, specifically T cells.
- Primed T cells homing to the respiratory tract are capable of conferring protection and accelerating bacterial clearance.
- Specific antibodies do not appear to play a major role in the enhanced pulmonary clearance of NTHI after mucosal immunization.
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