Protective effects of inosine on urinary bladder function in rats with partial bladder outlet obstruction

Area of Science:

• Urology
• Pharmacology
• Biochemistry

Background:

• Partial bladder outlet obstruction (PBOO) in rats leads to significant bladder dysfunction and oxidative stress.
• Understanding the molecular mechanisms underlying PBOO is crucial for developing effective therapeutic strategies.

Purpose of the Study:

• To investigate the potential protective effects of inosine on bladder function, nerve density, and oxidative damage in a rat model of PBOO.
• To determine the dose-dependent efficacy of inosine in mitigating PBOO-induced pathological changes.

Main Methods:

• Adult male Sprague-Dawley rats underwent partial bladder outlet obstruction (PBOO) and were treated with varying doses of inosine or saline.
• Functional bladder capacity was assessed via cystometry, and bladder tissues were analyzed for contractile responses, neuronal marker (PGP 9.5) density, superoxide dismutase (SOD) activity, and thiobarbituric acid reactive substances (TBARS) levels.

Main Results:

• Inosine administration demonstrated dose-dependent improvements in bladder contractility and reduced residual urine volume post-PBOO.
• Inosine treatment significantly increased PGP 9.5-positive nerve density and enhanced SOD activity while reducing TBARS levels, indicating reduced oxidative stress.
• Protective effects on contractile responses to field stimulation and carbachol were observed, with KCl responses requiring higher inosine doses.

Conclusions:

• Inosine exhibits significant protective effects against PBOO-induced bladder dysfunction in rats.
• Inosine ameliorates oxidative damage and restores nerve density in the obstructed bladder, suggesting its therapeutic potential.