Related Experiment Videos
A test tube method for quantitation of hemoglobin A2 using DE 52 cellulose
Hemoglobin
|January 1, 1977
Summary
A new test tube method accurately quantifies Hemoglobin A2 (Hb A2) using microcolumn chromatography. This simple, rapid technique allows for high-volume daily testing of Hb A2 levels.
Area of Science:
- Biochemistry
- Clinical Chemistry
- Hematology
Background:
- Quantification of Hemoglobin A2 (Hb A2) is crucial for diagnosing hemoglobinopathies.
- Existing methods for Hb A2 quantitation can be time-consuming or require specialized equipment.
Purpose of the Study:
- To describe a simplified, cost-effective test tube method for Hb A2 quantitation.
- To adapt the microcolumn chromatographic technique for routine laboratory use.
Main Methods:
- Utilized microgranular DEAE cellulose (DE 52) equilibrated with a specific glycine-KCN buffer (pH 7.25).
- Separated Hb A2 from Hemoglobin A (Hb A) and Hemoglobin F (Hb F) based on differential binding to the resin.
- Hb A2 remained in the supernatant, allowing for its direct quantitation.
Main Results:
- The method demonstrated simplicity, speed, and accuracy.
- One technician can process at least 50 samples per day.
- Applicable to various sample types: whole blood, hemolysates, and blood on filter paper.
Conclusions:
- The described test tube method provides a practical and efficient approach for Hb A2 quantitation.
- This technique is suitable for high-throughput screening and diagnosis of hemoglobin disorders.