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Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells

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Published on: August 5, 2016

Brucella abortus activates human neutrophils.

Astrid Zwerdling¹, M Victoria Delpino, Karina A Pasquevich

¹Instituto de Estudios de la Inmunidad Humoral (CONICET), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires (UBA), Buenos Aires, Argentina.

Microbes and Infection

|April 21, 2009

Summary

Brucella abortus lipoproteins directly activate human neutrophils, promoting inflammation and tissue damage in brucellosis. This study highlights the role of these lipoproteins in the disease

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Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells

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ATAC-Seq Library Preparation of Murine Bone Marrow-Derived Neutrophils

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Published on: January 3, 2025

Area of Science:

Immunology
Microbiology
Pathogenesis

Background:

Human brucellosis involves bacterial persistence and inflammation.
Neutrophils are key inflammatory cells in brucellosis-affected tissues.
Brucella abortus outer membrane lipoproteins (Omp) have inflammatory properties.

Purpose of the Study:

To investigate the effect of Brucella abortus and its lipoproteins on neutrophil functions.
To determine if Brucella lipoproteins contribute to brucellosis pathogenesis by activating neutrophils.

Main Methods:

Assessing neutrophil activation markers (CD35, CD11b, CD62L) and IL-8 secretion upon B. abortus exposure.
Evaluating the impact of B. abortus lipoprotein Omp19 (L-Omp19) on neutrophil functions, including oxidative burst, migration, and survival.
Comparing the effects of lipidated versus unlipidated L-Omp19.

Main Results:

B. abortus induced neutrophil activation, characterized by increased CD35/CD11b and decreased CD62L expression, along with IL-8 secretion.
Lipidated B. abortus Omp19 (L-Omp19) mimicked B. abortus-induced neutrophil activation.
L-Omp19 enhanced neutrophil oxidative burst, migration, and prolonged survival.

Conclusions:

Brucella lipoproteins possess pro-inflammatory properties.
Direct activation of neutrophils by Brucella lipoproteins contributes to localized tissue injury and inflammation in human brucellosis.
Brucella lipoproteins are implicated in the pathogenesis of human brucellosis.