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Planning a serial dilution test with multiple dilutions
1Food and Drug Administration, Center for Applied Nutrition and Food Safety, 5100 Paint Branch Parkway, College Park, MD 20740, USA. robert.blodgett@fda.hhs.gov
Abstract:
The dilutions used in a serial dilution test determine which concentrations it can estimate well. Two criteria help to select how many and which dilutions to use. The first criterion is a low probability of outcomes with either all growth or all non-growth tubes at the concentrations of interest. The second criterion considers how far the estimated concentration (MPN) is likely to be from the actual concentration.
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