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[Interleukin 2 promotes the expression of immunoglobulin G in a cervical cancer cell line HeLaS3]
Tian Lu1, Xiao-hui Zhu, Shi-qing Liu
1Department of Immunology, Peking University School of Basic Medical Sciences, Beijing 100191, China.
Objective:
To analyze the effect of interleukin 2 (IL-2) on immunoglobulin G(IgG) expression in cervical cancer cell line HeLaS3.
Methods:
By immunofluorescence-flow cytometry(FCM), we detected membrane and cytoplasmic IgG expression variation of HeLaS3 after IL-2 stimulation. By Western blot, we detected IgG expression variation of HeLaS3 after IL-2 stimulation. Meanwhile, by RT-PCR with primers designed to amplify four subclasses of IgG, we detected Gamma transcript variation in HeLaS3 after IL-2 stimulation at mRNA level.
Results:
The result of immunofluorescence-FACS showed that after IL-2 stimulation for 48 hours, membrane and cytoplasmic IgG expression elevated in HeLaS3. The result of Western blot showed after IL-2 stimulation for 48 hours, IgG expression elevated in HeLaS3. Meanwhile, the result of RT-PCR showed that Gamma1 transcript mildly elevated after IL-2 stimulation for 48 hours.
Conclusion:
IL-2 can promote IgG expression in cervical cancer cell line HeLaS3.
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