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Updated: Jun 23, 2026

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Detection of Bacteria Using Fluorogenic DNAzymes
Published on: May 28, 2012
Fluorescence resonance energy transfer between an anionic conjugated polymer and a dye-labeled lysozyme aptamer for
1Department of Chemical & Biomolecular Engineering, National University of Singapore, 4 Engineering Drive 4, Singapore.
Abstract:
Surface charge switch of a 6-carboxyfluorescein (FAM) labeled lysozyme aptamer from negative to positive upon lysozyme binding triggers fluorescence resonance energy transfer (FRET) between an anionic conjugated polymer and the aptamer, which provides a convenient method for lysozyme detection in biological media with high sensitivity and selectivity.
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