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Related Experiment Video

Updated: Jun 23, 2026

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
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Published on: August 9, 2019

Ligand-regulated peptide aptamers.

Russell A Miller1

  • 1University of Pennsylvania, Philadelphia, PA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|April 21, 2009
PubMed
Summary
This summary is machine-generated.

We developed a novel ligand-regulated peptide (LiRP) scaffold for controlling peptide aptamer interactions with small molecules. This system enables precise regulation of aptamer-target binding using rapamycin.

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Published on: March 21, 2018

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Protein Engineering

Background:

  • Peptide aptamers are powerful tools for molecular recognition, selected via high-throughput screening.
  • Current methods lack precise control over aptamer-target interactions.
  • Scaffold proteins are essential for displaying peptide aptamers.

Purpose of the Study:

  • To develop a novel peptide aptamer scaffold enabling small-molecule control over aptamer-target interactions.
  • To engineer a ligand-regulated peptide (LiRP) scaffold using FKBP12, FRB, and GST domains.
  • To present a protocol for creating, selecting, and screening ligand-regulated peptide aptamers.

Main Methods:

  • Construction of a peptide aptamer plasmid library.
  • Selection of peptide aptamers using the LiRP scaffold in a yeast two-hybrid system.
  • Screening for ligand-regulated interactions mediated by rapamycin binding.

Main Results:

  • Successful development of the LiRP scaffold integrating FKBP12, FRB, and GST domains.
  • Demonstration of rapamycin-induced inhibition of the randomizable linker region interaction.
  • Establishment of a protocol for generating and selecting ligand-regulated peptide aptamers.

Conclusions:

  • The LiRP scaffold offers a new paradigm for inducible control of peptide aptamer activity.
  • This system provides enhanced specificity and regulation in molecular interaction studies.
  • The developed protocol facilitates the creation of tunable molecular tools for biological research.