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A Rapid High-throughput Method for Mapping Ribonucleoproteins (RNPs) on Human pre-mRNA
Published on: December 2, 2009
Nucleic acid pool preparation and characterization
Shawn K Piasecki1, Bradley Hall, Andrew D Ellington
1Department of Chemistry and Biochemistry, University of Texas at Austin, Austin, TX, USA.
Methods in Molecular Biology (Clifton, N.J.)
|April 21, 2009
Summary
This protocol details preparing nucleic acid pools of known complexity for applications like aptamer selection. It simplifies tracking pool size and complexity for researchers using random sequence nucleic acids.
Area of Science:
- Molecular Biology
- Biochemistry
- Genetics
Background:
- Random sequence nucleic acid pools are crucial for selecting functional molecules like aptamers and ribozymes.
- While pool synthesis is manageable, accurately determining pool complexity and size presents a challenge for researchers.
Purpose of the Study:
- To provide a protocol for preparing nucleic acid pools with a defined and known complexity.
- To offer a method for simplifying the tracking of nucleic acid pool size and complexity.
Main Methods:
- The protocol outlines step-by-step procedures for the preparation of nucleic acid pools.
- Focuses on methods to accurately determine and control the complexity of the synthesized pool.
Main Results:
- Successful preparation of nucleic acid pools with a known complexity.
- A simplified approach to managing and tracking the characteristics of nucleic acid pools.
Conclusions:
- The described protocol effectively addresses the challenge of determining nucleic acid pool complexity.
- This method aids researchers in efficiently utilizing random sequence nucleic acid pools in various selection applications.
