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Related Experiment Video

Updated: Jun 23, 2026

Protocols for Robust Herbicide Resistance Testing in Different Weed Species
10:52

Protocols for Robust Herbicide Resistance Testing in Different Weed Species

Published on: July 2, 2015

Herbicide resistance screening assay.

Joan M Peterson1

  • 1BASF, 2901 S Loop Dr Ste 3800, Ames, IA 50010-8638, USA, joan.peterson@basf.com.

Methods in Molecular Biology (Clifton, N.J.)
|April 21, 2009
PubMed
Summary

Herbicide resistance screening in transgenic maize aids in assessing gene inheritance and linkage. This method also helps detect DNA silencing, ensuring accurate genetic analysis for crop improvement.

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Area of Science:

  • Plant Biotechnology
  • Molecular Genetics
  • Agricultural Science

Background:

  • Transgenic maize often utilizes herbicide resistance genes (Bar or Pat) as markers.
  • Assessing transgene inheritance and linkage is crucial for breeding programs.
  • Understanding DNA silencing is important for reliable genetic trait expression.

Purpose of the Study:

  • To evaluate herbicide resistance screening as a method for assessing gene inheritance and linkage in transgenic maize.
  • To investigate the utility of combined herbicide screening and PCR for studying transgene linkage.
  • To explore the application of these methods in detecting DNA silencing.

Main Methods:

  • Herbicide resistance screening assay on transgenic maize populations.
  • Polymerase Chain Reaction (PCR)-based screening of seedling DNA for marker and transgene presence.
  • Simultaneous phenotypic scoring (herbicide resistance) and genotypic analysis (PCR).
  • Non-destructive screening of seeds for phenotypic traits prior to planting.

Main Results:

  • Herbicide screening accurately determined the presence and inheritance ratios of phosphinothricin acetyltransferase genes (Bar/Pat).
  • Combined herbicide screening and PCR successfully identified linkage and potential breakdown of linkage between marker and transgene.
  • The methodology allowed for the evaluation of DNA silencing for both herbicide resistance and the gene of interest.

Conclusions:

  • Herbicide resistance screening is a versatile tool for genetic analysis in transgenic maize.
  • Integrated molecular and phenotypic screening enhances the reliability of genetic studies.
  • These methods provide insights into gene expression stability and potential genetic silencing.

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Last Updated: Jun 23, 2026

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