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Updated: Jun 23, 2026

Western Blotting: Sample Preparation to Detection
07:45

Western Blotting: Sample Preparation to Detection

Published on: October 14, 2010

Western blotting : remembrance of past things

W Neal Burnette1

  • 1Molecular Pharmaceutics Corporation, 85423 Dudley Drive, Chapel Hill, NC, 27517, USA. burnetten@iname.com

Methods in Molecular Biology (Clifton, N.J.)
|April 21, 2009
PubMed

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Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
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Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
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