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Related Concept Videos

Western Blotting01:15

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.
Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as  cells...
Southern Blot02:57

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...
SDS-PAGE01:27

SDS-PAGE

Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...

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Related Experiment Video

Updated: Jun 23, 2026

Immunoblot Analysis
16:01

Immunoblot Analysis

Published on: June 20, 2008

Introduction to protein blotting.

Biji T Kurien1, R Hal Scofield

  • 1Oklahoma Medical Research Foundation, 825 NE 13th Street, OK 73104, Oklahoma City, USA. biji-kurien@omrf.org

Methods in Molecular Biology (Clifton, N.J.)
|April 21, 2009
PubMed
Summary

Protein blotting is crucial for detecting low-abundance proteins after electrophoresis. This technique has significantly evolved since 1979, offering diverse methods for protein transfer from gels to membranes.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Immunology

Background:

  • Protein blotting is essential for immunodetection of proteins, especially those present in low abundance.
  • The foundational protocol for protein transfer from gels to membranes was established in 1979.
  • Significant advancements have been made in protein blotting techniques since its inception.

Purpose of the Study:

  • To review the evolution of protein blotting techniques.
  • To highlight the importance of protein blotting in scientific research.
  • To present the variety of methods available for protein transfer.

Main Methods:

  • Review of historical and modern protein blotting protocols.
  • Analysis of different protein transfer methods.

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Last Updated: Jun 23, 2026

Immunoblot Analysis
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Immunoblot Analysis

Published on: June 20, 2008

Western Blotting: Sample Preparation to Detection
07:45

Western Blotting: Sample Preparation to Detection

Published on: October 14, 2010

Western Blotting Using the Invitrogen NuPage Novex Bis Tris MiniGels
22:45

Western Blotting Using the Invitrogen NuPage Novex Bis Tris MiniGels

Published on: August 22, 2007

  • Discussion of immunodetection strategies.

    • Main Results:

    • Protein blotting has undergone substantial evolution since 1979.
    • Numerous methods now exist for efficient protein transfer.
    • The technique remains critical for detecting low-abundance proteins.

    Conclusions:

    • Protein blotting is a vital tool in molecular biology and biochemistry.
    • The diversity of available methods allows for optimized protein detection.
    • Continued innovation in protein blotting enhances scientific discovery.