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Southern Blot

Southern Blot

Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...

Western Blotting

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.

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Related Experiment Video

Updated: Jun 23, 2026

Western Blotting: Sample Preparation to Detection

Western Blotting: Sample Preparation to Detection

Published on: October 14, 2010

Slice blotting.

¹Monell Chemical Senses Center, 3500 Market St, Philadelphia, PA, 19104-3308, USA. loweg@monell.org

Methods in Molecular Biology (Clifton, N.J.)

|April 21, 2009

Summary

This summary is machine-generated.

Slice blotting is a novel technique to map extracellular signaling molecules from living tissue slices. This method visualizes secreted substances trapped on a membrane using immunoblotting for spatial distribution analysis.

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Area of Science:

Biochemistry
Molecular Biology
Cell Biology

Background:

Extracellular signaling molecules play crucial roles in tissue development and function.
Understanding the spatial distribution of these molecules is essential for studying biological processes.
Existing techniques may have limitations in resolving localized signaling events within tissue slices.

Purpose of the Study:

To introduce and describe the slice blotting technique.
To demonstrate its utility in analyzing secreted molecules from tissue explants.
To provide a method for visualizing the spatial patterns of extracellular signaling.

Main Methods:

Thin slices of living tissue are prepared.
Slices are placed onto a membrane designed to capture secreted substances.
The trapped substances (antigens) on the membrane are detected using immunoblotting techniques.

Main Results:

The technique successfully records the spatial distribution of extracellular signaling molecules.
Visualizations reveal patterns of secreted substances diffusing from the tissue slices.
Immunoblotting allows for the identification and localization of specific membrane-bound antigens.

Conclusions:

Slice blotting is an effective method for studying the spatial dynamics of secreted signaling molecules.
This technique offers valuable insights into cell-to-cell communication and tissue microenvironments.
Slice blotting provides a powerful tool for biochemical and molecular analyses in tissue engineering and developmental biology.