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Semi-dry protein transfer and immunodetection of P-selectin using an antibody to its C-terminal tag

Padmaja Mehta1

  • 1Cardiovascular Biology Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, 73104, USA. Padmaja-Mehta@omrf.org

Methods in Molecular Biology (Clifton, N.J.)
|April 21, 2009
PubMed

P-selectin is a multidomain glycoprotein expressed on activated endothelial cells. We previously expressed a recombinant form of P-selectin containing only its N-terminal lectin and EGF domains in CHO-K1 cells and showed that these two domains are sufficient to mediate ligand binding. We have now expressed the same construct in CHO-Lec1 cells that make truncated glycans. The uniform glycosylation in these cells should make it easier to crystallize this protein.

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