A brief review of other notable protein detection methods on blots

Several methods have been used for detecting proteins on membranes. These include the use of quantum dot luminescent labels, oxyblot immunochemical detection, polymer immunocomplexes, "coupled" probing approach, in situ renaturation of proteins for detecting enzyme activities in crude or purified preparations, immunochromatographic assay, western-phosphatase assay, and the use of Congo red dye, a cosmetic color named Alta, Pro-Q Emerald 488 dye, or amine-reactive dye in combination with alkaline phosphatase and horseradish peroxidase-antibody conjugates for the simultaneous trichromatic fluourescence detection of proteins. Several methods have been used to improve the detection of proteins on membranes, including glutaraldehyde treatment of nitrocellulose blots, elimination of keratin artifacts in immunoblots probed with polyclonal antibodies, and the washing of immunoblots with excessive water and manipulation of Tween-20 in wash buffer. These methods are briefly reviewed in this chapter.