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Related Experiment Video

Updated: Jun 23, 2026

Loss-of-Function Approach in the Embryonic Chick Retina by Using Tol2 Transposon-Mediated Transgenic Expression of Artificial microRNAs

Loss-of-Function Approach in the Embryonic Chick Retina by Using Tol2 Transposon-Mediated Transgenic Expression of Artificial microRNAs

Published on: May 18, 2022

Transient and stable transgenesis using tol2 transposon vectors.

Hiroshi Kikuta¹, Koichi Kawakami

¹Division of Molecular and Developmental Biology, National Institute of Genetics, 1111 Yata, Mishima, Shizuoka, 411-8540, Japan.

Methods in Molecular Biology (Clifton, N.J.)

|April 21, 2009

Summary

Transgenesis in zebrafish is enhanced by Tol2 transposable element vectors. These vectors simplify gene function studies by enabling easy cloning and manipulation for both transient and stable transgenic fish.

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Last Updated: Jun 23, 2026

Loss-of-Function Approach in the Embryonic Chick Retina by Using Tol2 Transposon-Mediated Transgenic Expression of Artificial microRNAs

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An Efficient In Vitro Transposition Method by a Transcriptionally Regulated Sleeping Beauty System Packaged into an Integration Defective Lentiviral Vector

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Published on: January 12, 2018

Area of Science:

Molecular Biology
Genetics
Developmental Biology

Background:

Transgenesis is crucial for understanding gene and genome function in model organisms.
The Tol2 transposable element facilitates transgenesis in the zebrafish model vertebrate.
Previous methods allowed analysis of transgene function in transient and stable transgenic fish.

Purpose of the Study:

To describe the features of advanced Tol2 transposon vectors for zebrafish transgenesis.
To provide protocols for performing transgenesis in zebrafish using these vectors.
To highlight the utility of Gateway-compatible transposon vectors for rapid gene cloning.

Main Methods:

Utilizing Tol2 transposable element-based vectors for zebrafish transgenesis.
Employing transposon vectors with minimal DNA sequences for easier manipulation.
Leveraging Gateway recombination technology for efficient cloning into transposon vectors.

Main Results:

Identification of cis-sequences essential for Tol2 element transposition.
Development of versatile transposon vectors with minimal sequences.
Creation of Gateway-compatible transposon vectors enabling rapid foreign sequence cloning.

Conclusions:

Advanced Tol2 transposon vectors significantly improve the ease and efficiency of zebrafish transgenesis.
These vectors facilitate robust gene function studies through simplified cloning and manipulation.
The described protocols enable researchers to generate transient and stable transgenic zebrafish effectively.