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Related Experiment Video

Updated: Jun 23, 2026

Multi-Photon Time Lapse Imaging to Visualize Development in Real-time: Visualization of Migrating Neural Crest Cells in Zebrafish Embryos

Multi-Photon Time Lapse Imaging to Visualize Development in Real-time: Visualization of Migrating Neural Crest Cells in Zebrafish Embryos

Published on: August 9, 2017

Multiple embryo time-lapse imaging of zebrafish development.

Leah Herrgen¹, Christian Schröter, Lola Bajard

¹Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, D*resden, 01307, Germany.

Methods in Molecular Biology (Clifton, N.J.)

|April 21, 2009

Summary

This protocol details a time-lapse microscopy method for observing multiple embryos simultaneously. It enables precise tracking of tissue-scale morphogenetic movements and developmental processes under controlled conditions.

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Area of Science:

Developmental Biology
Cellular Dynamics
Microscopy Techniques

Background:

Understanding biological processes requires high-resolution temporal and spatial data.
Simultaneous observation of multiple embryos aids in statistical analysis of biological variability.

Purpose of the Study:

To describe a protocol for parallel time-lapse microscopy of multiple embryos.
To enable precise documentation of developmental and cellular processes.

Main Methods:

Utilizes time-lapse microscopy for parallel observation of multiple embryos.
Employs tightly controlled environmental conditions for consistent development.
Achieves temporal resolution in the minute range for extended observation periods (hours to days).

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A Layered Mounting Method for Extended Time-Lapse Confocal Microscopy of Whole Zebrafish Embryos

A Layered Mounting Method for Extended Time-Lapse Confocal Microscopy of Whole Zebrafish Embryos

Published on: January 14, 2020

A Versatile Mounting Method for Long Term Imaging of Zebrafish Development

A Versatile Mounting Method for Long Term Imaging of Zebrafish Development

Published on: January 26, 2017

Related Experiment Videos

Last Updated: Jun 23, 2026

Multi-Photon Time Lapse Imaging to Visualize Development in Real-time: Visualization of Migrating Neural Crest Cells in Zebrafish Embryos

Multi-Photon Time Lapse Imaging to Visualize Development in Real-time: Visualization of Migrating Neural Crest Cells in Zebrafish Embryos

Published on: August 9, 2017

A Layered Mounting Method for Extended Time-Lapse Confocal Microscopy of Whole Zebrafish Embryos

A Layered Mounting Method for Extended Time-Lapse Confocal Microscopy of Whole Zebrafish Embryos

Published on: January 14, 2020

A Versatile Mounting Method for Long Term Imaging of Zebrafish Development

A Versatile Mounting Method for Long Term Imaging of Zebrafish Development

Published on: January 26, 2017

Main Results:

The method is well-suited for tracking tissue-scale morphogenetic movements.
Allows for comparative studies between wild-type and mutant embryos or chemically treated groups.
Facilitates investigation of temperature-dependent biological processes.

Conclusions:

This parallel time-lapse microscopy protocol offers a robust method for studying developmental dynamics.
It enhances the ability to quantify biological variability and compare experimental conditions.
The technique is valuable for diverse applications in developmental and cellular biology research.