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Maxam-Gilbert Sequencing

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...

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Related Experiment Video

Updated: Jun 23, 2026

Assessment of DNase Activity by Ratiometric Fluorescence Resonance Energy Transfer

Assessment of DNase Activity by Ratiometric Fluorescence Resonance Energy Transfer

Published on: July 25, 2025

DNase I footprinting.

Benoît Leblanc¹, Tom Moss

¹Département de Biologie, Faculté des Sciences, Université de Sherbrooke, 2500 Boulevard de l'Université, Sherbrooke, QC, Canada J1K 2R1. Benoit.Leblanc@usherbrooke.ca

Methods in Molecular Biology (Clifton, N.J.)

|April 21, 2009

Summary

DNAse I footprinting identifies DNA-protein interactions by comparing DNA digestion patterns with and without proteins. This method reveals protected DNA regions and binding site affinities, crucial for understanding gene regulation.

Area of Science:

Molecular Biology
Biochemistry
Genetics

Background:

Protein binding to DNA can block nuclease access, especially with large enzymes like DNAse I.
Understanding DNA-protein interactions is fundamental to gene regulation and cellular processes.

Purpose of the Study:

To describe the DNAse I footprinting method for studying DNA-protein interactions.
To explain how this technique reveals DNA protection and binding site characteristics.

Main Methods:

Comparing DNA fragmentation patterns after partial digestion by DNAse I in the presence and absence of specific proteins.
Analyzing gel electrophoresis results to identify regions of DNA protected from or showing altered cleavage by DNAse I.

Main Results:

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DNAse I footprinting generates distinct fragment patterns when proteins are bound to DNA.
Protected regions (footprints) indicate protein binding sites.
Variations in cleavage can highlight differential protein affinities and multiple binding sites.

Conclusions:

DNAse I footprinting is a valuable technique for mapping DNA-protein interactions.
The method provides insights into the location, number, and relative affinities of DNA-binding proteins.