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Updated: Jun 23, 2026

Advanced Confocal Microscopy Techniques to Study Protein-protein Interactions and Kinetics at DNA Lesions
Published on: November 12, 2017
Ultraviolet crosslinking of DNA protein complexes via 8-azidoadenine
Rainer Meffert1, Klaus Dose, Gabriele Rathgeber
1Johannes Gutenberg-Universitêt, Fachbereich Chemie, Pharmazie und Geowissenschaften, Institut fü Biochemie, 55099 Mainz, Germany.
Abstract:
The synthesis of 8-azido-2'-deoxyadenosine-5'-triphosphate is described. The photoreactive dATP analog was characterized by thin layer chromatography and UV spectroscopy. Its photoreactivity upon UV irradiation was studied. After incorporation of this dATP analog by nick translation into DNA containing the tet operator sequence the investigation of the interactions between tet operator DNA and Tet repressor becomes possible. Photocrosslinking of protein to DNA was demonstrated by the reduced migration of the DNA protein crosslinks in SDS polyacrylamide gel electrophoresis.
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