Transfusion results of platelet concentrates using different cell separators

Area of Science:

• Hematology
• Transfusion Medicine
• Biomedical Engineering

Background:

• Bone marrow aplasia/hypoplasia necessitates regular platelet transfusions.
• Platelet concentrate (PC) quality and survival impact transfusion efficacy.
• Automated blood cell separators (e.g., AS-104, CS-3000) are used for PC collection.

Purpose of the Study:

• To compare the in vivo survival and recovery of fresh human leukocyte antigen (HLA)-selected platelet concentrates collected using AS-104 versus CS-3000 separators.
• To evaluate the impact of storage conditions on platelet concentrate efficacy.

Main Methods:

• 115 patients with bone marrow aplasia/hypoplasia received 567 transfusions of fresh HLA-selected platelet concentrates.
• Platelet counts were monitored daily pre- and up to seven days post-transfusion.
• Post-transfusional increments per 10(11) platelets transfused were calculated to assess in vivo recovery and survival.

Main Results:

• Platelets from the AS-104 showed comparable initial recovery but significantly better survival compared to those from the CS-3000.
• These findings align with previous in vitro studies reporting better function and morphology for AS-104 derived platelets.
• Limited evaluation of AS-104 PCs stored in teflon bags for up to five days showed unfavorable results compared to CS-3000 PCs in polyolefine bags, potentially due to bag geometry.

Conclusions:

• The AS-104 separator yields platelets with superior in vivo survival in patients requiring transfusions for bone marrow aplasia/hypoplasia.
• Improved platelet survival from AS-104 may allow for prolonged transfusion intervals.
• Storage bag geometry may influence the efficacy of stored platelet concentrates.