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Active oxygen transforms murine myeloid progenitor cells in vitro
D R Crawford1, J S Greenberger
1Department of Radiation Oncology, University of Massachusetts Medical Center, Worcester 01655.
International Journal of Cancer
|November 11, 1991
Summary
Cytotoxic levels of active oxygen (AO) can transform mouse myeloid progenitor cells into factor-independent cells, leading to tumor formation in mice. Non-cytotoxic AO concentrations did not induce this transformation.
Area of Science:
- Cell Biology
- Toxicology
- Carcinogenesis
Background:
- Active oxygen (AO) species are naturally occurring and can act as carcinogens.
- Understanding AO's role in cellular transformation is crucial for cancer research.
Purpose of the Study:
- To investigate the effect of active oxygen on the transformation of mouse myeloid progenitor cells.
- To determine if cytotoxic or non-cytotoxic concentrations of AO induce factor independence (FI).
Main Methods:
- Utilized anchorage-independent colony formation in methylcellulose assay.
- Exposed cells to cytotoxic and non-cytotoxic concentrations of t-butylhydroperoxide, hydrogen peroxide, and menadione.
- Injected transformed cells into mice to assess tumorigenicity.
Main Results:
- Cytotoxic concentrations of AO induced factor independence (FI) in cells, with t-butylhydroperoxide being the most potent.
- Single exposures to cytotoxic AO were sufficient for transformation.
- Transformed cells uniformly produced tumors when injected into mice.
Conclusions:
- Cytotoxic active oxygen is a potent inducer of cellular transformation and tumorigenicity.
- Transformed cells appear to rely on paracrine mechanisms for growth.