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Updated: Jun 23, 2026

A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model
Published on: February 6, 2018
Centromere-specific multicolour fluorescence in situ hybridization on human spermatocyte I and II metaphases
1Departament de Biologia Cel.lular, Fisiologia i Immunologia, Facultat de Medicina, Universitat Autònoma de Barcelona, 08193 Bellaterra, Spain.
Background:
Most meiotic studies in metaphase spermatocytes have been carried out with classic cytogenetic techniques. The aim of this work was to adjust the centromere-specific multicolour fluorescence in situ hybridization (cenM-FISH) procedure to spermatocyte metaphases I and II in order to improve the identification of meiotic chromosome abnormalities.
Methods:
A total of 168 spermatocytes I and 66 spermatocytes II from two fertile males have been studied using cenM-FISH.
Results:
The mean frequency of meiotic abnormalities (synaptic, numerical and structural errors) found in metaphases I and II was 22.1 and 3.0%, respectively. The cenM-FISH technique has not only enabled the individual identification of chromosomes involved in meiotic disorders, but also increased the number of analysable cells, principally at metaphase II stage.
Conclusions:
CenM-FISH is a useful tool to study the meiotic chromosomal disorders and mechanisms leading to chromosomally abnormal spermatozoa.
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