Simultaneous determination of voriconazole and posaconazole concentrations in human plasma by high-performance liquid
Kathrin Kahle1, Peter Langmann, Diana Schirmer
1Department of Internal Medicine II, Division of Infectious Diseases, University of Wuerzburg Medical Center, Josef-Schneider Strasse 2, Wuerzburg, Germany.
Abstract:
A simple, sensitive, and selective high-performance liquid chromatographic method for the simultaneous determination of voriconazole and posaconazole concentrations in human plasma was developed and validated. Quantitative recovery following liquid-liquid extraction with diethyl ether was achieved. Linearity ranged from 0.10 to 20.0 microg/ml for voriconazole and from 0.05 to 10.0 microg/ml for posaconazole. The intra- and interday coefficients of variation were less than 8.5%, and the lower limits of quantitation were <0.05 microg/ml.
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