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Related Concept Videos

Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.

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Related Experiment Video

Updated: Jun 23, 2026

High-Throughput Total Internal Reflection Fluorescence and Direct Stochastic Optical Reconstruction Microscopy Using a Photonic Chip
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Published on: November 16, 2019

Diffuse optical reflection tomography using continuous wave illumination.

X Cheng, D Boas

    Optics Express
    |April 22, 2009
    PubMed
    Summary
    This summary is machine-generated.

    Diffuse optical reflection tomography reconstructs absorption images using continuous-wave light measurements. While simple and fast for pseudo-3D imaging with one light source, quantitative 3D imaging requires multiple sources.

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    Area of Science:

    • Biomedical optics
    • Medical imaging
    • Photonics

    Background:

    • Diffuse optical reflection tomography (DORT) is a non-invasive imaging technique.
    • It utilizes the scattering and absorption properties of biological tissues.
    • Continuous-wave (CW) measurements are commonly used for DORT.

    Purpose of the Study:

    • To reconstruct absorption images from CW diffuse optical measurements.
    • To evaluate the feasibility of pseudo-3D imaging using a single light source.
    • To identify requirements for quantitative 3D imaging.

    Main Methods:

    • Employed diffuse optical reflection tomography (DORT).
    • Utilized continuous-wave (CW) measurements of diffuse light.
    • Reconstructed pseudo-3D absorption images from a semi-infinite medium.

    Main Results:

    • Successfully reconstructed absorption images.
    • The imaging algorithm proved simple and fast.
    • Pseudo-3D images were achievable with a single light source.

    Conclusions:

    • The developed algorithm enables rapid pseudo-3D diffuse optical tomography.
    • Quantitative 3D imaging necessitates algorithm modifications.
    • Incorporating multi-source measurements is a key future direction.