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Updated: Jun 23, 2026

An Open-Source Framework for Mass Calculation of Antibody-Based Therapeutic Molecules
Published on: June 16, 2023
Bioanalysis of recombinant proteins and antibodies by mass spectrometry
Eric Ezan1, Mathieu Dubois, François Becher
1CEA, IBITECS, Service de Pharmacologie et d'Immunoanalyse, 91191 Gif-sur-Yvette, France. eric.ezan@cea.fr
Abstract:
In recent years, biotechnologically-derived drugs have been a major focus of research and development in the pharmaceutical industry. Their pharmacokinetics and pharmacokinetic/pharmacodynamic relationships impact every stage of the development process and require their assessment in the circulation in preclinical species and in humans. To this end, immunoassays are a reference, but standardisation remains an issue owing to the restricted pattern of antibody specificity and interference with endogenous components. As an alternative, we report here analytical strategies involving liquid chromatography coupled to mass spectrometry (LC-MS) for the accurate quantification of therapeutic proteins and antibodies in biological fluids.
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