Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dithioether Inhibitors of Bacterial RNA Polymerase-Sigma Factor Interactions Exhibit In Vivo Efficacy against MRSA.

Journal of medicinal chemistry·2025
Same author

Spatial dynamics of brain development and neuroinflammation.

Nature·2025
Same author

Pinoresinol diglucoside alleviates ovariectomy-induced osteoporosis by modulating the "Microbiota-gut-bone" axis.

Biochemical and biophysical research communications·2025
Same author

Spatial metabolic gradients in the liver and small intestine.

Nature·2025
Same author

Munc13-4 mediates tumor immune evasion by regulating the sorting and secretion of PD-L1 via exosomes.

Nature communications·2025
Same author

Potential mechanism of ginger <i>(Zingiber Officinale Roscoe)</i> in alleviating osteoporosis.

Frontiers in pharmacology·2025

Related Experiment Video

Updated: Jun 23, 2026

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster
09:39

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

Published on: August 21, 2014

MicroRNAs - powerful repression comes from small RNAs.

Cong Ma1, YuFei Liu, Lin He

  • 1Division of Cell and Developmental Biology, MCB Department, University of California at Berkeley, Berkeley, CA 94720-3200, USA.

Science in China. Series C, Life Sciences
|April 22, 2009
PubMed
Summary

MicroRNAs (miRNAs) are small RNAs regulating gene expression. Research is advancing our understanding of miRNA biogenesis and gene silencing mechanisms, crucial for complex gene networks.

More Related Videos

mirMachine: A One-Stop Shop for Plant miRNA Annotation
06:16

mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools
09:29

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

Published on: August 21, 2019

Related Experiment Videos

Last Updated: Jun 23, 2026

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster
09:39

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

Published on: August 21, 2014

mirMachine: A One-Stop Shop for Plant miRNA Annotation
06:16

mirMachine: A One-Stop Shop for Plant miRNA Annotation

Published on: May 1, 2021

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools
09:29

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

Published on: August 21, 2019

Area of Science:

  • Molecular Biology
  • Genetics
  • RNA Biology

Background:

  • MicroRNAs (miRNAs) are a major class of non-coding RNAs.
  • They regulate gene expression post-transcriptionally.
  • Thousands of miRNA genes have been identified across metazoans.

Purpose of the Study:

  • To explore the biogenesis and function of microRNAs.
  • To elucidate the mechanisms of miRNA-mediated gene silencing.
  • To understand the role of miRNAs in gene regulatory networks.

Main Methods:

  • Genetic approaches
  • Biochemical analyses
  • Computational methods

Main Results:

  • Key molecular machinery for miRNA biogenesis identified.
  • Recent findings illuminate miRNA generation and repression mechanisms.
  • miRNAs act as endogenous small RNAs for genome-wide gene silencing.

Conclusions:

  • MicroRNA gene silencing is a fundamental aspect of gene regulation.
  • Understanding miRNA mechanisms deepens insights into complex gene regulatory networks.
  • Further research promises to add new dimensions to RNA biology.