Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Protein-protein Interfaces02:04

Protein-protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...
Targets for Drug Action: Overview01:26

Targets for Drug Action: Overview

Drugs target macromolecules to modify ongoing cellular processes. Primary drug targets include receptors, ion channels, transporters, and enzymes.
Receptors are either membrane-spanning or intracellular proteins, which upon binding a ligand, get activated and transmit the signal downstream to elicit a response. Drugs bind receptors, either mimicking the action of endogenous ligands or blocking the receptor activity to bring about a modified response. Nearly 35% of approved drugs target the G...
Drug Discovery: Overview01:26

Drug Discovery: Overview

Drug discovery is a multifaceted process involving extensive screening, testing, and optimization of lead compounds to identify potential new drugs for therapeutic use. It combines several approaches, including screening large numbers of natural products, chemical modification of known active molecules, identification of new drug targets, and rational design based on biological mechanisms and drug-receptor structure. These approaches are carried out in both academic research laboratories and...
Protein-Drug Binding: Determination Methods01:22

Protein-Drug Binding: Determination Methods

Determining protein-drug binding can be achieved through indirect and direct methods, each providing valuable insights into the interaction between proteins and drugs.
Indirect methods involve isolating the bound drug from its free form in biological samples such as blood, serum, or plasma. These techniques aim to measure the percentage of drugs bound to proteins. Equilibrium dialysis is a commonly used method where the free drug concentration at equilibrium is measured by separating the bound...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Incidence and Morphological Predictors of Intrastent Coronary Thrombus After Drug-Eluting Stent Implantation (from a Multicenter Registry).

The American journal of cardiology·2015
Same author

Overexpression of wildtype EGFR is tumorigenic and denotes a therapeutic target in non-small cell lung cancer.

Oncotarget·2015
Same author

Shared Genetic Etiology between Type 2 Diabetes and Alzheimer's Disease Identified by Bioinformatics Analysis.

Journal of Alzheimer's disease : JAD·2015
Same author

Collective punishment is more effective than collective reward for promoting cooperation.

Scientific reports·2015
Same author

Application of novel catalytic-ceramic-filler in a coupled system for long-chain dicarboxylic acids manufacturing wastewater treatment.

Chemosphere·2015
Same author

Coexistence of Scattering Enhancement and Suppression by Plasmonic Cavity Modes in Loaded Dimer Gap-Antennas.

Scientific reports·2015
Same journal

Physiological characteristics of the primitive CO(2) concentrating mechanism in PEPC transgenic rice.

Science in China. Series C, Life sciences·2010
Same journal

An efficient method of constructing homologous recom binant baculovirus with PCR-amplified fragments.

Science in China. Series C, Life sciences·2010
Same journal

Muscle injection of rAAV/mFIX to secrete clotting factor IX corrects the hemorrhagic tendencies in hemophilia B mice.

Science in China. Series C, Life sciences·2010
Same journal

Chicken retinal ganglion cells response characteristics: multi-channel electrode recording study.

Science in China. Series C, Life sciences·2010
Same journal

Behavioral modification in choice process ofDrosophila.

Science in China. Series C, Life sciences·2010
Same journal

Studies on gene structure, enzymatic activity and regulatory mechanism of acetohydroxy acid isomeroreductase from G2 pea.

Science in China. Series C, Life sciences·2010
See all related articles

Related Experiment Video

Updated: Jun 23, 2026

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions
08:31

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions

Published on: December 1, 2020

Identifying drug-target proteins based on network features.

MingZhu Zhu1, Lei Gao, Xia Li

  • 1School of Biomedical Engineering, Capital Medical University, Beijing 100069, China.

Science in China. Series C, Life Sciences
|April 22, 2009
PubMed
Summary
This summary is machine-generated.

Drug-target proteins exhibit higher connectivity within the human protein-protein interaction (PPI) network. Analyzing these network properties aids in understanding drug mechanisms and identifying novel drug targets.

Related Experiment Videos

Last Updated: Jun 23, 2026

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions
08:31

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions

Published on: December 1, 2020

Area of Science:

  • Bioinformatics
  • Systems Biology
  • Pharmacology

Background:

  • Proteins function within complex cellular networks, not in isolation.
  • Understanding drug-target interactions within these networks is crucial for drug mechanism elucidation.
  • Limited characterization exists for the topological features of drug-target proteins in the human interactome.

Purpose of the Study:

  • To analyze and compare the topological features of drug-target proteins within the human protein-protein interaction network.
  • To investigate the network properties of proteins targeted by drugs.
  • To identify potential novel drug targets based on network topology.

Main Methods:

  • Mapping drug targets from DrugBank onto the human protein-protein interaction network.
  • Analyzing five topological indices for drug-target proteins.
  • Comparing these indices against the entire protein interactome and non-drug-target sets.
  • Ranking proteins based on identified network features.

Main Results:

  • Drug-target proteins demonstrate higher connectivity and faster communication within the protein-protein interaction network.
  • A significant proportion of top-ranked proteins in the network are identified as drug targets.
  • The study identified known and potential novel drug targets through network analysis.

Conclusions:

  • Drug-target proteins possess distinct topological characteristics within the human interactome.
  • Network analysis provides valuable insights into drug action mechanisms.
  • This approach can effectively identify and prioritize potential drug targets for further investigation.