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Histone Modification

Histone Modification

The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...

Histone Modification

Histone Modification

The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone deacetylase,...

Chromatin Immunoprecipitation- ChIP

Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...

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Related Experiment Video

Updated: Jun 23, 2026

An Acetyl-Click Chemistry Assay to Measure Histone Acetyltransferase 1 Acetylation

An Acetyl-Click Chemistry Assay to Measure Histone Acetyltransferase 1 Acetylation

Published on: January 26, 2024

Histone deacetylase activity assay.

Zhigang Yuan¹, Natalie Rezai-Zadeh, Xiaohong Zhang

¹H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL, USA.

Methods in Molecular Biology (Clifton, N.J.)

|April 22, 2009

Summary

This summary is machine-generated.

This article details methods for assaying histone deacetylase (HDAC) enzymatic activity. It covers techniques for measuring HDACs, which are crucial for maintaining histone acetylation balance and modifying proteins.

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Assays for Validating Histone Acetyltransferase Inhibitors

Assays for Validating Histone Acetyltransferase Inhibitors

Published on: August 6, 2020

Area of Science:

Biochemistry
Molecular Biology
Epigenetics

Background:

Histone deacetylases (HDACs) are enzymes that remove acetyl groups from histones.
HDACs play a role in maintaining histone acetylation equilibrium.
HDACs can also modify non-histone proteins.

Purpose of the Study:

To provide updated, easy-to-follow approaches for assaying HDAC enzymatic activities.
To focus on specific techniques for measuring the activity of these enzymes.

Main Methods:

The article focuses on the assay of enzymatic activities.
Specific techniques and approaches are detailed.

Main Results:

The article provides up-to-date methods for HDAC assays.
Techniques are presented in an easy-to-follow manner.

Conclusions:

Accurate assaying of HDAC activity is essential for understanding their biological roles.
The provided methods facilitate research into HDAC function and potential therapeutic targeting.