Related Experiment Video
Updated: Jun 23, 2026

Proteomic Profiling of Macrophages by 2D Electrophoresis
Published on: November 4, 2014
Glycoproteomic analysis by two-dimensional electrophoresis
Mary Ann Comunale1, Anand Mehta
1Department of Microbiology and Immunology, Drexel University, 700 East Butler, Doylestown, PA 18901, USA.
Changes in cancer glycosylation, like increased fucosylation in hepatocellular carcinoma (HCC), are linked to tumor progression. New targeted glycoproteomics methods now allow comprehensive identification of fucosylated proteins in cancer.
Area of Science:
- Biochemistry
- Oncology
- Proteomics
Background:
- Aberrant N-linked glycosylation is a hallmark of cancer development.
- Increased oligosaccharide branching correlates with metastasis and tumor progression in various cancers.
- Elevated core fucosylation is specifically linked to hepatocellular carcinoma (HCC) development.
Purpose of the Study:
- To identify the specific proteins associated with altered N-linked glycosylation in cancer.
- To investigate the fucosylated proteome in hepatocellular carcinoma (HCC).
- To demonstrate the utility of targeted glycoproteomics for comprehensive fucosylated protein identification.
Main Methods:
- Utilized sensitive glycan analysis and advanced proteomic technologies.
- Applied targeted glycoproteomics to analyze the fucosylated proteome.
- Focused on a human liver cancer cell line for method demonstration.
Main Results:
- Enabled comprehensive identification of fucosylated proteins in a cancer cell line.
- Laid the groundwork for identifying unknown fucosylated proteins in cancer.
- Demonstrated a generally applicable method for fucosylated proteome analysis.
Conclusions:
- Targeted glycoproteomics is a powerful approach to identify cancer-associated fucosylated proteins.
- This method can be widely applied to various cancer types and biological contexts.
- Understanding the fucosylated proteome is crucial for cancer research and biomarker discovery.
More Related Videos
12:34Two-dimensional Gel Electrophoresis Coupled with Mass Spectrometry Methods for an Analysis of Human Pituitary Adenoma Tissue Proteome
Published on: April 2, 2018
10:51Consensus Brain-derived Protein, Extraction Protocol for the Study of Human and Murine Brain Proteome Using Both 2D-DIGE and Mini 2DE Immunoblotting
Published on: April 10, 2014
Related Concept Videos
Two-dimensional Gel Electrophoresis
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as cells...
SDS-PAGE
A variation of gel electrophoresis, termed polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...
Electrophoresis: Overview
There...
Capillary Electrophoresis: Applications
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...