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Tandem Mass Spectrometry

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Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...

Peptide Identification Using Tandem Mass Spectrometry

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Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
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MALDI-TOF Mass Spectrometry

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Related Experiment Video

Updated: Jun 23, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Serum proteomics using mass spectrometry.

Brian L Hood¹, David E Malehorn, Thomas P Conrads

¹Clinical Proteomics Facility, University of Pittsburgh Cancer Institute, Pittsburgh, PA, USA.

Methods in Molecular Biology (Clifton, N.J.)

|April 22, 2009

Summary

This study presents mass spectrometry methods for discovering novel cancer biomarkers in serum. These advanced techniques aim to improve early cancer detection, diagnosis, and treatment strategies.

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Area of Science:

Oncology
Biomarker Discovery
Translational Cancer Research

Background:

Limited clinical application of discovered tumor markers necessitates new approaches.
Cancer genomics and proteomics are transforming molecular diagnosis and medicine.
Improved molecular tools are needed for cancer diagnosis, prognosis, and treatment.

Purpose of the Study:

To describe detailed methods for mass spectrometry-based biomarker discovery in serum/plasma.
To optimize the identification and quantitation of low-abundance cancer biomarkers.
To advance the development of novel cancer therapies and prevention strategies.

Main Methods:

Utilized two complementary mass spectrometry approaches: MALDI-TOF and RPLC-MS/MS.
Incorporated abundant protein depletion and sample fractionation.
Focused on serum/plasma samples for biomarker discovery.

Main Results:

Detailed methods and workflows for mass spectrometry-based biomarker discovery are presented.
Optimization strategies for identifying and quantifying low-abundance species were employed.
The study lays the groundwork for improved molecular diagnostic and therapeutic tools.

Conclusions:

Mass spectrometry-based approaches offer powerful tools for cancer biomarker discovery.
Advanced workflows enhance the identification of crucial low-abundance biomarkers.
This research contributes to the development of more effective cancer therapies and prevention.