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High-throughput analysis of serum antigens using sandwich ELISAs on microarrays

Shannon L Servoss1, Rachel Gonzalez, Susan Varnum

  • 1Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|April 22, 2009
PubMed

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Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.