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Updated: Jun 23, 2026

Optimization for Sequencing and Analysis of Degraded FFPE-RNA Samples

Optimization for Sequencing and Analysis of Degraded FFPE-RNA Samples

Published on: June 8, 2020

RNA preparation and characterization for gene expression studies.

Michael Stangegaard¹

¹University of Copenhagen, Copenhagen, Denmark.

Methods in Molecular Biology (Clifton, N.J.)

|April 22, 2009

Summary

This summary is machine-generated.

Gene expression analysis is now possible from single cells. Amplified RNA can be reverse transcribed and fluorescently labeled for DNA microarray hybridization, enabling detailed transcriptome studies.

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Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

Area of Science:

Molecular Biology
Genomics
Biotechnology

Background:

Understanding gene expression is crucial for biological insights.
Technological advancements allow for gene expression studies using minimal starting material.

Purpose of the Study:

To describe a method for gene expression analysis from single cells.
To enable detailed transcriptome profiling with limited samples.

Main Methods:

Linear amplification of messenger RNA (mRNA) from a single cell.
Reverse transcription of amplified RNA.
Fluorescent labeling of RNA for hybridization.

Main Results:

Achieved over 10(6)-fold amplification of single-cell mRNA.
Generated stable targets for DNA microarray analysis.

Conclusions:

Single-cell gene expression profiling is feasible with current technologies.
Amplified and labeled RNA provides a robust platform for transcriptome analysis.