Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Chromatin Immunoprecipitation- ChIP

Chromatin Immunoprecipitation- ChIP

Chromatin immunoprecipitation, or ChIP, is an antibody-based technique used to identify sites on DNA that bind to transcription factors of interest or histone proteins. It also helps determine the type of histone modifications such as acetylation, phosphorylation, or methylation.
Types of ChIP
ChIP can be divided into two types - X-ChIP and N-ChIP. X-ChIP involves in vivo cross-linking of histones and regulatory proteins to DNA, fragmenting the DNA by sonication, and isolating the protein-DNA...

Immunoprecipitation

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...

DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Fermentation in breadmaking: enhancing digestibility and nutritional value for gastrointestinal health.

Frontiers in nutrition·2026

Same author

Interplay between cohesin and TORC1 links chromosome segregation and gene expression to environmental changes.

eLife·2026

Same author

Performance and viability of yeast (Saccharomyces cerevisiae) during baking: An integrative electrical resistance oven (ERO) approach.

Food chemistry·2026

Same author

Vitamin D Nutritional Status in the Middle East and North Africa Region: A Systematic Review and Meta-analysis.

Current developments in nutrition·2025

Same author

Disease-specific epigenetic deregulation of enhancers, transposons, and polycomb targets in acute promyelocytic leukemia.

Genome medicine·2025

Same author

The nutritional contribution and relationship with health of bread consumption: a narrative review.

Critical reviews in food science and nutrition·2024

Search research articles

Related Experiment Video

Updated: Jun 23, 2026

Chromatin Immunoprecipitation of Murine Brown Adipose Tissue

Chromatin Immunoprecipitation of Murine Brown Adipose Tissue

Published on: November 21, 2018

Chromatin immunoprecipitation using microarrays.

Mickaël Durand-Dubief¹, Karl Ekwall

¹Karolinska Institute /NOVUM, Huddinge, Sweden.

Methods in Molecular Biology (Clifton, N.J.)

|April 22, 2009

Summary

This summary is machine-generated.

Chromatin immunoprecipitation followed by microarray analysis (ChIP-chip) identifies genome-wide protein-DNA interactions in vivo. This method uses immunoaffinity purification and DNA microarrays to detect specific DNA binding sites for proteins.

More Related Videos

Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing (ChIP-seq)

Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing (ChIP-seq)

Published on: April 19, 2013

Efficient Chromatin Immunoprecipitation using Limiting Amounts of Biomass

Efficient Chromatin Immunoprecipitation using Limiting Amounts of Biomass

Published on: May 1, 2013

Related Experiment Videos

Last Updated: Jun 23, 2026

Chromatin Immunoprecipitation of Murine Brown Adipose Tissue

Chromatin Immunoprecipitation of Murine Brown Adipose Tissue

Published on: November 21, 2018

Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing (ChIP-seq)

Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing (ChIP-seq)

Published on: April 19, 2013

Efficient Chromatin Immunoprecipitation using Limiting Amounts of Biomass

Efficient Chromatin Immunoprecipitation using Limiting Amounts of Biomass

Published on: May 1, 2013

Area of Science:

Molecular Biology
Genomics
Biochemistry

Background:

Chromatin immunoprecipitation (ChIP) is essential for studying protein-DNA interactions.
Understanding these interactions is key to deciphering gene regulation and cellular processes.

Purpose of the Study:

To describe the ChIP-chip technique for genome-wide identification of protein-DNA interactions.
To highlight its utility as a powerful tool for in vivo investigations.

Main Methods:

ChIP-chip integrates chromatin immunoprecipitation with DNA microarray analysis.
Key steps include chemical cross-linking, cell lysis, DNA fragmentation, and immunoaffinity purification.
Enriched DNA fragments are amplified, labeled, and hybridized to microarrays for signal detection.

Main Results:

The method enables the identification of specific DNA regions bound by proteins across the genome.
Enriched signals on microarrays pinpoint in vivo protein-DNA association sites.

Conclusions:

ChIP-chip is a robust technique for comprehensive genome-wide mapping of protein-DNA interactions.
It provides valuable insights into the functional roles of proteins in DNA binding and regulation.