Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 23, 2026

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry
05:53

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Published on: June 21, 2018

Target preparation for genotyping specific genes or gene segments.

Jesper Petersen1, Lena Poulsen, Martin Dufva

  • 1Technical University of Denmark, Jyllinge, Denmark.

Methods in Molecular Biology (Clifton, N.J.)
|April 22, 2009
PubMed
Summary

This study presents three reliable methods for amplifying single-stranded DNA targets, crucial for microarray hybridization. These protocols incorporate biotin labels, enabling flexible visualization strategies for enhanced experimental outcomes.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Osteoarthritis and analgesic consumption in haemochromatosis HFE C282Y homozygotes with normal or low iron parameters.

Nature communications·2026
Same author

Risk of fractures according to iron parameters and hemochromatosis <i>HFE</i> genotype in 142,146 general population individuals.

Haematologica·2026
Same author

Incidentally Detected Splenomegaly and Risk of Hematologic Cancer and Liver Disease.

JAMA oncology·2026
Same author

ChipNMR: Hyperpolarized NMR for Noninvasive Metabolic Flux Analysis in Perfused Microfluidic Chips.

Analytical chemistry·2026
Same author

Effect of Low-Load Blood-Flow Restricted Training Versus Heavy Slow Resistance Training in Unilateral Patellar Tendinopathy: A Randomized Clinical Trial.

Scandinavian journal of medicine & science in sports·2025
Same author

Characteristics and predictors of clinical outcome in patients with pleural effusions caused by heart, liver and renal failure: results from the ERS International Multicentre Pleural Research Collaborative (IMPACT) registry.

ERJ open research·2025

Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • Single-stranded nucleic acid generation is vital for hybridization-based assays like oligonucleotide microarrays.
  • Existing methods for producing single-stranded DNA and in vitro transcribed RNA have limitations.
  • Efficient target amplification is essential for sensitive and reliable molecular diagnostics.

Purpose of the Study:

  • To describe three robust and reproducible methods for single-stranded target amplification.
  • To enable the incorporation of biotin labels during target amplification for versatile detection.
  • To provide protocols adaptable for purified genomic DNA or pre-amplified DNA samples.

Main Methods:

  • Development and validation of three distinct protocols for target amplification.

More Related Videos

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
07:24

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing

Published on: February 10, 2023

gDNA Enrichment by a Transposase-based Technology for NGS Analysis of the Whole Sequence of BRCA1, BRCA2, and 9 Genes Involved in DNA Damage Repair
08:15

gDNA Enrichment by a Transposase-based Technology for NGS Analysis of the Whole Sequence of BRCA1, BRCA2, and 9 Genes Involved in DNA Damage Repair

Published on: October 6, 2014

Related Experiment Videos

Last Updated: Jun 23, 2026

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry
05:53

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry

Published on: June 21, 2018

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
07:24

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing

Published on: February 10, 2023

gDNA Enrichment by a Transposase-based Technology for NGS Analysis of the Whole Sequence of BRCA1, BRCA2, and 9 Genes Involved in DNA Damage Repair
08:15

gDNA Enrichment by a Transposase-based Technology for NGS Analysis of the Whole Sequence of BRCA1, BRCA2, and 9 Genes Involved in DNA Damage Repair

Published on: October 6, 2014

  • Incorporation of biotin labels into amplified target molecules.
  • Utilizing biotin/streptavidin chemistry for subsequent detection and visualization.

    • Main Results:

    • Successful amplification of single-stranded DNA targets using the described methods.
    • Demonstrated incorporation of biotin labels within the amplified targets.
    • Showcased the flexibility of biotin/streptavidin chemistry for various visualization strategies.

    Conclusions:

    • The presented methods offer robust and efficient means for single-stranded target generation.
    • Biotin labeling provides a versatile platform for diverse detection techniques in microarray applications.
    • These protocols enhance the utility of oligonucleotide microarrays by improving target preparation and detection flexibility.