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Updated: Jun 23, 2026
Preparation Of Gushukang (GSK) Granules for In Vivo and In Vitro Experiments
Published on: May 9, 2019
[Study on preparation and quantity determination of Scrophularia ningpoensis dispensing granules]
Zhengrui Niu1, Rufeng Wang, Yu Jing
1Division of Pharmacognosy, School of Pharmaceutical Sciences, Peking University, Beijing 100083, China.
Objective:
To develop the preparation method and to determine the quantity of effective constituents of Scrophularia ningpoensis dispensing granules.
Method:
Nine experiments were carried out through L9 (3(4)) orthogonal design. The contents of harpagoside and cinnamic acid were determined by HPLC method.
Result:
The optimal extraction process was as follows: the slices were soaked in water in the ratio of 1:8 for 0.5 h, then they were decocted for 2 h. For the second time, 6 times of water was added and sustained for 1.5 h. Harpagoside and cinnamic acid were linear within the ranges of 0.0776-1.552 microg (r=0.9999) and 0.01446-0.4339 microg (r=0.9999), respectively. The average recoveries were 100.4% (RSD 1.98%, n=6) and 98.27% (RSD1.35%, n=6), respectively. Ten batches of granules were determined.
Conclusion:
The extraction process is scientific and the determination method is suitable for quality control of Scrophularia ningpoensis dispensing granules.
